LOCUS       sequence-204455-        6639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS driven green to red switch reporter for B3 recombinase 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA
  TITLE     Presynaptic Inhibition Selectively Gates Auditory Transmission to 
            the Brainstem Startle Circuit.
  JOURNAL   Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 
            10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.
  PUBMED    30078569
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.06.020"; journalName: "Curr Biol"; date: 
            "2018-08-20- 20"; volume: "28"; issue: "16"; pages: "2527-2535.e8"
COMMENT     Sequence Label: UAS:bloSwitch
FEATURES             Location/Qualifiers
     source          1..6639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1609..1629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2394..2413)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2459..2514
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2513..2532)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4243..4260)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4243..4259)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4252..4274)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4468..4487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4587..4609
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4647..4665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4733..4837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4838..4906,4907..5698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5056..5075)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5869..6457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6358..6377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6611..6628
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg
      301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact
      361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag
      421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg
      481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg
      541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag
      601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg
      661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac
      721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
      781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc
      841 ctccacacga attcgatact aaggatcctt gtttgtgagt atgatgttaa atataagcaa
      901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa
      961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg
     1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa
     1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc
     1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct
     1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt
     1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt
     1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt
     1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc
     1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca
     1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gcgtaaagat
     1561 atcggttgct taagaataag taattcttaa gcaaccgcca tcatggcccc aaagaagaaa
     1621 cgcaaagtga tggtctcaaa gggagaggaa ctgtttactg gagtcgtgcc tatcctggtg
     1681 gagctggatg gagatgtgaa tggacacaag ttctctgtgt ctggagaggg agagggagat
     1741 gctacatatg gaaagctgac actgaagttc atctgtacaa caggaaagct gcctgtgccc
     1801 tggcctacac tggtgacaac attcacatat ggagtgcagt gttttgctag ataccctgac
     1861 cacatgaagc agcatgactt cttcaagtct gctatgcctg agggatatgt gcaggagaga
     1921 acaatcttct tcaaggatga tggaaactac aagacaagag ctgaggtgaa gtttgaggga
     1981 gacacactgg tgaacagaat tgagctgaag ggaattgact tcaaggagga tggaaacatc
     2041 ctgggacaca agctggagta caactacaac agccacaagg tgtacatcac agctgacaag
     2101 cagaagaatg gaatcaaggt gaacttcaag acaagacaca acattgagga tggatctgtg
     2161 cagctggctg accactacca gcagaacaca cctattggag atggacctgt gctgctgcct
     2221 gacaaccact acctgagcac acagtctgct ctgagcaagg accctaatga gaagagagac
     2281 cacatggtgc tgctggagtt tgtgacagct gctggaatca cacatggaat ggatgagctg
     2341 tacaagtgaa ctagtaatta agtctcagcc accgttcctc tcgagcctct agatcttttt
     2401 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2461 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2521 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2581 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     2641 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     2701 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     2761 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     2821 cctcttctct tatgaagatc ggttgcttaa gaataagtaa ttcttaagca accgccatca
     2881 tgggatgcat taaaagtaaa agaaaagata acctgaacga tgatgagatg gtgagcaaag
     2941 gagaggaact gatcaaggag aacatgcaca tgaagctgta catggaggga acagtgaaca
     3001 accaccactt caagtgtaca tctgagggag agggaaagcc ttatgaggga acacagacaa
     3061 tgagaatcaa ggtggtggag ggaggacctc tgccttttgc ttttgacatc ctggctacca
     3121 gcttcatgta tggaagcaga acattcatca accacacaca gggaatccct gacttcttca
     3181 agcagagctt ccctgaggga ttcacatggg agagagtgac aacatatgag gatggaggag
     3241 tgctgacagc tacacaggac acaagcctcc aggatggatg tctgatctac aatgtgaaga
     3301 tcagaggagt gaacttccct agcaatggac ctgtgatgca gaagaagaca ctgggatggg
     3361 aggctaacac agagatgctg taccctgctg atggaggact ggagggaaga acagacatgg
     3421 ctctgaagct ggtgggagga ggacacctga tctgtaactt caagacaaca tacagaagca
     3481 agaagcctgc taagaacctg aagatgcctg gagtgtacta tgtggaccac agactggaga
     3541 gaatcaagga ggctgacaag gagacatatg tggagcagca tgaggtggct gtggctagat
     3601 actgtgacct gcctagcaag ctgggacaca agctgaatgg aatggatgag ctgtacaagt
     3661 gactcgagac tagtaattaa gtctcagcca ccgttaactg aacatgtcaa aacctgtgga
     3721 gactgttgag atttgatgtt ctgaaaagat aaagcctata aataaaatgt tgcccaaatt
     3781 tcctgcctga tgtttttctt tgtctttgct acatggcttt gctgctcgga tcggctcact
     3841 ctgtgtatgc cacgttcact ttgtactctc cttctcacgg taggtttatt atttttagat
     3901 gtgcagttag tttctgtgaa ataacacacc acacactgat attgtctgtg cattgacttg
     3961 gtgagtgcac attgtttttg atcttgacat atttatattt gattgatcag gtgaactgtg
     4021 tgaatctaaa gtgctccata cagatgttct gcattgaaaa tattctcatt ttattagtgg
     4081 aagtgagtgt atgccagcgg ccgcggtacc ctgcagtcta gacatttcag ttgacgaaga
     4141 caaacaaagt tctgttgtga ctatgggggg ggggggcgcc tggggatggt ctcgcccggg
     4201 gagtaattca gggtagaacc gccactgcct ttagcgacat tcactggccg tcgttttaca
     4261 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4321 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4381 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4441 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4501 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4561 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4621 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4681 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4801 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4861 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4921 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4981 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5041 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5101 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5161 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5221 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5281 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5341 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5401 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5461 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5521 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5581 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5641 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5701 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6541 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6601 gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
