LOCUS       sequence-204456-        5664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS driven variant of iGluSnFR for zebrafish expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA
  TITLE     Presynaptic Inhibition Selectively Gates Auditory Transmission to 
            the Brainstem Startle Circuit.
  JOURNAL   Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 
            10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.
  PUBMED    30078569
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.06.020"; journalName: "Curr Biol"; date: 
            "2018-08-20- 20"; volume: "28"; issue: "16"; pages: "2527-2535.e8"
COMMENT     Sequence Label: UAS:iGluSnfr.zf1
FEATURES             Location/Qualifiers
     source          1..5664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1904..1925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2031..2052)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2292..2311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2517..2546
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2550..2693
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKP"
     primer_bind     complement(3268..3285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3268..3284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3277..3299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3493..3512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3612..3634
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3672..3690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3758..3862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3863..3931,3932..4723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4081..4100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4894..5482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5383..5402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5636..5653
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg
      301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact
      361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag
      421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg
      481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg
      541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag
      601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg
      661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac
      721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
      781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc
      841 ctccacacga attcgctagc gccaccatgt tcgctctggg aatctatctg tgggaaacta
      901 tcgtgttctt cagcctggct gccagccagc aggctgctag aagcgccgca ggcagcacgc
      961 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtgcctt
     1021 tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
     1081 ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
     1141 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt gaatgtggtt
     1201 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
     1261 tcggtacgcg cctgttgacc aaaaagggtg gcgatatcaa agattttgcc aacctgaaag
     1321 acaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
     1381 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
     1441 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgtgctgctg gccggtgaac
     1501 gtgcgaaagc gaagaaacca gacaactggg aaatcgtcgg caagccgcag tctcaggagg
     1561 cctacggttg tatgttgcgt aaagatgatc cgcagttcaa aaagctgatg gatgacacca
     1621 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataagtgg ttcaaaaatc
     1681 caattctggt gagccacaac gtctatatca tggccgacaa gcagaggaac ggcatcaagg
     1741 cgaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc
     1801 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1861 cccagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1921 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag
     1981 ggagcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2041 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca
     2101 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2161 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2221 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca
     2281 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2341 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2401 ggcataagct ggagtacaac tttaacaacc ccctgaacat gaatttcgaa ctgtcagacg
     2461 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaaactgcag gtcgacgaac
     2521 aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg
     2581 tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg gccctggtgg
     2641 tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag ccacctagta
     2701 attaagtctc agccaccgtt aactgaacat gtcaaaacct gtggagactg ttgagatttg
     2761 atgttctgaa aagataaagc ctataaataa aatgttgccc aaatttcctg cctgatgttt
     2821 ttctttgtct ttgctacatg gctttgctgc tcggatcggc tcactctgtg tatgccacgt
     2881 tcactttgta ctctccttct cacggtaggt ttattatttt tagatgtgca gttagtttct
     2941 gtgaaataac acaccacaca ctgatattgt ctgtgcattg acttggtgag tgcacattgt
     3001 ttttgatctt gacatattta tatttgattg atcaggtgaa ctgtgtgaat ctaaagtgct
     3061 ccatacagat gttctgcatt gaaaatattc tcattttatt agtggaagtg agtgtatgcc
     3121 agcggccgcg gtaccctgca gtctagacat ttcagttgac gaagacaaac aaagttctgt
     3181 tgtgactatg gggggggggg gcgcctgggg atggtctcgc ccggggagta attcagggta
     3241 gaaccgccac tgcctttagc gacattcact ggccgtcgtt ttacaacgtc gtgactggga
     3301 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3421 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     3481 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3541 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3601 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3661 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3721 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3841 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3901 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3961 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4021 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4081 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4141 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4201 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4261 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4321 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4381 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4441 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4501 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4561 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4621 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4681 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4741 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4801 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4921 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4981 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5041 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5101 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5161 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5221 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5281 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5341 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5401 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5461 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5521 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5581 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5641 gtcagtgagc gaggaagcgg aaga
//
