LOCUS       sequence-204519-         456 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGAL1 in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD-pGAL1 (01-11)
FEATURES             Location/Qualifiers
     source          1..456
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..446
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    5..122
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     417..440
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg
       61 cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc
      121 cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt
      181 aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg
      241 cgattagttt tttagcctta tttctggggt aattaatcag cgaagcggtg atttttgatc
      301 tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt
      361 ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata
      421 tacctctata ctttaacgtc aaggagaaaa aaccca
//
