LOCUS       sequence-20452-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(26..150)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(158..174)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(158..174)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(180..443)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(263..286)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gcctgggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
       61 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      121 ttctcgttca gcttttttgt acaaacttgt gatgggggat ccactagttc tagaatccgt
      181 cgaaactaag ttctggtgtt ttaaaactaa aaaaaagact aactataaaa gtagaattta
      241 agaagtttaa gaaatagatt tacagaatta caatcaatac ctaccgtctt tatatactta
      301 ttagtcaagt aggggaataa tttcagggaa ctggtttcaa cctttttttt cagctttttc
      361 caaatcagag agagcagaag gtaatagaag gtgtaagaaa atgagataga tacatgcgtg
      421 ggtcaattgc cttgtgtcat catttactcc aggcaggttg catcactcca ttgaggttgt
      481 gcccgttttt tgcctgtttg tgcccctgtt ctctgtagtt gcgctaagag aatggaccta
      541 tgaactgatg gttggtgaag aaaacaatat tttggtgctg ggattctttt tttttctgga
      601 tgccagctta aaaagcgggc tccattatat ttagtggatg ccaggaataa actgtt
//
