LOCUS       sequence-204527-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for xCas9(3.6)-BE4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X,
            Rees HA, Lin Z, Liu DR
  TITLE     Evolved Cas9 variants with broad PAM compatibility and high DNA 
            specificity.
  JOURNAL   Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
  PUBMED    29512652
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155."
COMMENT     Sequence Label: xCas9(3.6)-BE4
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             409..1095
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1192..5292
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.6
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVGEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLAKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5323..5571
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5602..5850
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5863..5883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5892..5909
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5932..5949)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5938..6162
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6233..6249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6246..6268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6257..6273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6281..6287,6288..6305,6306..6311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6464..6481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6635..7223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6715..6734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7394..8185,8186..8254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8017..8036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8255..8359)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8438..8457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8629..8877,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
      421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
      481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
      541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
      601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
      661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
      721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
      781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
      841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
      901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
      961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
     1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
     1081 gccaccgggt tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg
     1141 acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc tgacaagaag
     1201 tactccattg ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag
     1261 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
     1321 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
     1381 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
     1441 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
     1501 tttttggtgg gggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
     1561 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
     1621 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
     1681 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
     1741 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
     1801 ggagttgacg ccaaagcaat cctgagcgct aggctggcca aatcccggcg gctcgaaaac
     1861 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
     1921 tccctcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga taccaagctt
     1981 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
     2041 cagtacgcag accttttttt ggcggcaaaa aacctgtcag acgccattct gctgagtgat
     2101 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc
     2161 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
     2221 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
     2281 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
     2341 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact
     2401 ttcgacaatg gaatcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
     2461 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
     2521 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg
     2581 atgactcgca aatcagaaga gaccatcact ccctggaact tcgagaaagt cgtggataag
     2641 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
     2701 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
     2761 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagatcag
     2821 aagaaagcta ttgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
     2881 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
     2941 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
     3001 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
     3061 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
     3121 gacaaagtca tgaagcagct caaaaggcgc cgatatacag gatgggggcg gctgtcaaga
     3181 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
     3241 tccgatggat ttgccaaccg gaacttcatt cagttgatcc atgatgactc tctcaccttt
     3301 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc
     3361 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
     3421 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
     3481 cgagagaacc aaaccaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
     3541 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
     3601 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
     3661 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
     3721 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaacaga
     3781 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
     3841 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
     3901 cgaggtggcc tgtctgagtt ggataaagcc ggtttcatca aaaggcagct tgttgagaca
     3961 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
     4021 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
     4081 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
     4141 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
     4201 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
     4261 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
     4321 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
     4381 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
     4441 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggt
     4501 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
     4561 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
     4621 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
     4681 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
     4741 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
     4801 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgtgctgca gaaaggtaac
     4861 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
     4921 ctcaaggggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
     4981 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
     5041 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
     5101 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
     5161 aagtacttcg acactaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
     5221 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
     5281 ctcggtggag actctggtgg ttctggagga tctggtggtt ctactaatct gtcagatatt
     5341 attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag
     5401 gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac
     5461 gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct
     5521 tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct ctctggtggt
     5581 tctggaggat ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag
     5641 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg
     5701 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat
     5761 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat
     5821 agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa
     5881 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     5941 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6001 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6061 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6121 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6181 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6301 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8401 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
     8461 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     8521 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     8581 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     8641 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     8701 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     8761 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     8821 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//
