LOCUS       sequence-204531-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTPI1 in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD-pTPI1 (01-11)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        399..918
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
ORIGIN
        1 ctacgtatgg tcatttcttc ttcagattcc ctcatggaga aagtgcggca gatgtatatg
       61 acagagtcgc cagtttccaa gagactttat tcaggcactt ccatgatagg caagagagaa
      121 gacccagaga tgttgttgtc ctagttacac atggtattta ttccagagta ttcctgatga
      181 aatggtttag atggacatac gaagagtttg aatcgtttac caatgttcct aacgggagcg
      241 taatggtgat ggaactggac gaatccatca atagatacgt cctgaggacc gtgctaccca
      301 aatggactga ttgtgaggga gacataacta catagtgttt aaagattacg gatatttaac
      361 ttacttagaa taatgccatt tttttgagtt ataataatcc tacgttagtg tgagcgggat
      421 ttaaactgtg aggaccttaa tacattcaga cacttctgcg gtatcaccct acttattccc
      481 ttcgagatta tatctaggaa cccatcaggt tggtggaaga ttacccgttc taagactttt
      541 cagcttcctc tattgatgtt acacctggac accccttttc tggcatccag tttttaatct
      601 tcagtggcat gtgagattct ccgaaattaa ttaaagcaat cacacaattc tctcggatac
      661 cacctcggtt gaaactgaca ggtggtttgt tacgcatgct aatgcaaagg agcctatata
      721 cctttggctc ggctgctgta acagggaata taaagggcag cataatttag gagtttagtg
      781 aacttgcaac atttactatt ttcccttctt acgtaaatat ttttcttttt aattctaaat
      841 caatcttttt caattttttg tttgtattct tttcttgctt aaatctataa ctacaaaaaa
      901 cacatacata aactaaaa
//
