LOCUS       sequence-204535-        1613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G418R in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD-G418R (01-21)
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            129..1485
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        129..472
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             473..1282
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRRAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(540..559)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1288..1485
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1548..1581
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt ataatgtatg
       61 ctatacgaag ttattaggtc tagagatctg tttagcttgc ctcgtccccg ccgggtcacc
      121 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
      181 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
      241 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
      301 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
      361 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
      421 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
      481 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      541 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      601 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      661 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      721 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
      781 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      841 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
      901 cgtatttcgt cgcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      961 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     1021 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     1081 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     1141 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     1201 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1261 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     1321 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     1381 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     1441 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     1501 taacgccgcc atccagtgtc gaaaacgagc tctcgagaac ccttaatata acttcgtata
     1561 atgtatgcta tacgaagtta ttaggtgata tcagatccac tagtggccta tgc
//
