LOCUS       sequence-204536-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HygroBR in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD-HygroBR (01-21)
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            129..1704
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        129..472
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             473..1501
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVFDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAT
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      1507..1704
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1767..1800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt ataatgtatg
       61 ctatacgaag ttattaggtc tagagatctg tttagcttgc ctcgtccccg ccgggtcacc
      121 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
      181 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
      241 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
      301 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
      361 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
      421 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
      481 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
      541 cttcgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
      601 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
      661 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
      721 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
      781 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcaa tggatgcgat
      841 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
      901 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
      961 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     1021 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     1081 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgacgtt
     1141 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     1201 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     1261 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     1321 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     1381 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     1441 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     1501 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     1561 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     1621 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     1681 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg
     1741 aaaacgagct ctcgagaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
     1801 taggtgatat cagatccact agtggcctat gc
//
