LOCUS       sequence-204543-        3521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. solfataricus PCNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Iwata F, Hirakawa H, Nagamune T
  TITLE     Three proliferating cell nuclear antigen homologues from 
            Metallosphaera sedula form a head-to-tail heterotrimer.
  JOURNAL   Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588.
  PUBMED    27228945
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-27"; volume: "6:26588. doi"; pages: " 
            10.1038/srep26588"
COMMENT     Sequence Label: pMD-SsoPCNA2
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1056
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIGESLHACRSTLEDLLVIWIGMPWESHMMMKAK
                     VIDAVSFSYILRTVGDFLSEANFIVTKEGIRVSGIDPSRVVFLDIFLPSSYFEGFEVSQ
                     EKEIIGFKLEDVNDILKRVLKDDTLILSSNESKLTLTFDGEFTRSFELPLIQVESTQPP
                     SVNLEFPFKAQLLTITFADIIDELSDLGEVLNIHSKENKLYFEVIGDLSTAKVELSTDN
                     GTLLEASGADVSSSYGMEYVANTTKMRRASDSMELYFGSQIPLKLRFKLPQEGYGDFYI
                     APRADSGSGMPWNRIPGYRARIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRP
                     APIALPNSCAA"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(911..928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(911..927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(920..942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1135..1154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1254..1276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1314..1332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1400..1504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1505..1573,1574..2365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1723..1742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2536..3124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3025..3044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3278..3295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3412..3433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3448..3453,3454..3471,3472..3478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3486..3502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3491..3513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3510..3521,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3510..3521,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag ctatttaggt gacactatag gggaaagctt gcatgcctgc
       61 aggtcgactc tagaggatct actagtcata tggattggca tgccatggga atcccatatg
      121 atgatgaaag ccaaagtgat cgatgcagtt tctttcagtt acatcctgcg taccgtgggc
      181 gatttcctga gcgaagccaa ctttattgtg acgaaagaag gcattcgtgt tagtggtatc
      241 gatccgagcc gcgtggtttt tctggatatc ttcctgccga gctcttactt tgaaggcttc
      301 gaagttagcc aggaaaaaga aatcatcggt ttcaaactgg aagatgtgaa cgatattctg
      361 aaacgtgttc tgaaagatga taccctgatc ctgagtagca atgaatctaa actgaccctg
      421 acgtttgatg gtgaatttac ccgcagtttt gaactgccgc tgattcaggt ggaatctacg
      481 cagccgccga gtgttaatct ggaatttccg ttcaaagcgc agctgctgac cattacgttt
      541 gccgatatta tcgatgaact gagtgatctg ggcgaagtgc tgaacatcca tagcaaagaa
      601 aacaaactgt acttcgaagt gatcggtgat ctgagcaccg cgaaagttga actgtctacc
      661 gataacggca cgctgctgga agcgtctggt gccgatgtgt ctagtagcta tggcatggaa
      721 tacgttgcaa ataccacgaa aatgcgtcgc gcgagcgatt ctatggaact gtacttcggt
      781 agccagattc cgctgaaact gcgttttaaa ctgccgcagg aaggctacgg tgatttctac
      841 atcgcaccgc gcgcggattc gggatccggc atgccatgga atcggatccc cgggtaccga
      901 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      961 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1021 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggccc tgatgcggta
     1081 ttttctcctt acgcatctgt gcggtatttc acaccgcata tagtgcactc tcagtacaat
     1141 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     1201 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1261 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     1321 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     1381 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1441 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1501 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1561 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1621 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1681 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1741 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1801 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1861 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1921 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1981 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2041 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2101 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2161 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2221 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2281 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2341 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2401 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2461 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2521 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2581 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2641 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2701 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2761 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2821 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2881 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2941 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3001 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3061 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3361 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     3421 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     3481 tggaattgtg agcggataac aatttcacac aggaaacagc t
//
