LOCUS       sequence-204589-        6384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Weak affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.GFAP.SF-Venus-iGluSnFR.S72A
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(144..149,150..167,168..174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    182..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     187..209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     repeat_region   260..389
                     /label=AAV2 ITR
     regulatory      1141..1150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     1147..1206
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2175..2196
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2299..2320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2785..2814
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2818..2964
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2980..3568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3033..3053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3451..3462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3571..3587)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3572..3588)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3617..3738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3628..3647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3682..3701
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4117..4705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4197..4216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4876..5667,5668..5736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5737..5841)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5867..6322)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6004..6025)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6216..6235
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc mkgsmgyygg
       61 smsrmcwkkk ktcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacagga aacagctwkg rmmrkgmkww cgsygmgggc
      241 ctcttcgcta ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      301 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      361 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      421 atctacgtag ccatgctcta ggaagatcca acatatcctg gtgtggagta ggggacgctg
      481 ctctgacaga ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc
      541 aggcctttgt ctgcaagcag acctggcagc attgggctgg ccgcccccca gggcctcctc
      601 ttcatgccca gtgaatgact caccttggca cagacacaat gttcggggtg ggcacagtgc
      661 ctgcttcccg ccgcacccca gcccccctca aatgccttcc gagaagccca ttgagcaggg
      721 ggcttgcatt gcaccccagc ctgacagcct ggcatcttgg gataaaagca gcacagcccc
      781 ctaggggctg cccttgctgt gtggcgccac cggcggtgga gaacaaggct ctattcagcc
      841 tgtgcccagg aaaggggatc aggggatgcc caggcatgga cagtgggtgg caggggggga
      901 gaggagggct gtctgcttcc cagaagtcca aggacacaaa tgggtgaggg gagagctctc
      961 cccatagctg ggctgcggcc caaccccacc ccctcaggct atgccagggg gtgttgccag
     1021 gggcacccgg gcatcgccag tctagcccac tccttcataa agccctcgca tcccaggagc
     1081 gagcagagcc agagcaggtt ggagaggaga cgcatcacct ccgctgctcg cggggatccc
     1141 gccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     1201 actggtgaca gatctgccgc aggcagcacg ctggacaaaa tcgccaaaaa cggtgtgatt
     1261 gtcgtcggtc accgtgaatc ttcagtgcct ttctcttatt acgacaatca gcaaaaagtg
     1321 gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
     1381 aaaccggact tgcaggtaaa actgattccg attaccgcac aaaaccgtat tccactgctg
     1441 caaaacggca ctttcgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa
     1501 caggcggctt tctctgacac tattttcgtg gtcggtacgc gcctgttgac caaaaagggt
     1561 ggcgatatca aagattttgc caacctgaaa gacaaagccg tagtcgtcac ttccggcact
     1621 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
     1681 agcgccaaag atcacggtga ctctttccgc accctggaaa gcggtcgtgc cgttgccttt
     1741 atgatggatg acgtgctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
     1801 gaaatcgtcg gcaagccgca gtctcaggag gcctacggtt gtatgttgcg taaagatgat
     1861 ccgcagttca aaaagctgat ggatgacacc atcgctcagg tgcagacctc cggtgaagcg
     1921 gaaaaatggt ttgataagtg gttcaaaaat ccaattctgg tgagccacaa cgtctatatc
     1981 accgccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacgtggag
     2041 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2101 gtgctgctgc ccgacaacca ctacctgagc tatcagtccg tgctgagcaa agaccccaac
     2161 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2221 atggacgagc tgtacaaggg cggtaccgga gggagcatga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2341 gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gctcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg
     2461 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt taacaacccc
     2701 ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga accgaatgac
     2761 aaggcactga aactgcaggt cgacgaacaa aaactcatct cagaagagga tctgaatgct
     2821 gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg
     2881 gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc
     2941 atgctttggc agaagaagcc acgttagaag cttatcgata atcaacctct ggattacaaa
     3001 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3061 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3181 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3241 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3301 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3361 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3421 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3481 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3541 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgacc cgggcggccg
     3601 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3661 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3721 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3781 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     3841 gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa
     3901 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgactc
     3961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4861 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4921 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4981 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5041 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5101 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5161 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5221 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5281 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5341 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5401 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5461 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5521 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5581 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5641 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5701 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5761 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5821 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     5881 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5941 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6121 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6301 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     6361 cgatcggtgc gggcctcttc gcta
//
