LOCUS       sequence-204601-        6684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Weak affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.CAG.SF-Venus-iGluSnFR.S72A
FEATURES             Location/Qualifiers
     source          1..6684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..401
                     /label=chicken beta-actin promoter
     primer_bind     680..702
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     764..783
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     823..882
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1851..1872
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1975..1996)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2461..2490
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2494..2640
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2656..3244
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2709..2729)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3127..3138)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3287..3408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3298..3317)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3352..3371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3884..4472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3964..3983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4643..5434,5435..5503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5266..5285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5504..5608)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5634..6089)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5771..5792)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5983..6002
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6374..6395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6410..6415,6416..6433,6434..6440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     repeat_region   6526..6655
                     /label=AAV2 ITR
ORIGIN
        1 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
       61 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      121 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      181 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      241 gggngsgcgc gccaggcggg gcggggcggg gsgrggggsg gggsggggcg aggcggagag
      301 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      361 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      421 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      481 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
      541 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
      601 agggcccttt gtgcgggggg agcggctcgg ggctgtccgc ggggggacgg ctgccttcgg
      661 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
      721 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
      781 gtgctgtctc atcattttgg caaagaattg gatcccgcca ccatggagac agacacactc
      841 ctgctatggg tactgctgct ctgggttcca ggttccactg gtgacagatc tgccgcaggc
      901 agcacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca
      961 gtgcctttct cttattacga caatcagcaa aaagtggtgg gttactcgca ggattactcc
     1021 aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca ggtaaaactg
     1081 attccgatta ccgcacaaaa ccgtattcca ctgctgcaaa acggcacttt cgattttgaa
     1141 tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc tgacactatt
     1201 ttcgtggtcg gtacgcgcct gttgaccaaa aagggtggcg atatcaaaga ttttgccaac
     1261 ctgaaagaca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa
     1321 ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca cggtgactct
     1381 ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgt gctgctggcc
     1441 ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct
     1501 caggaggcct acggttgtat gttgcgtaaa gatgatccgc agttcaaaaa gctgatggat
     1561 gacaccatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga taagtggttc
     1621 aaaaatccaa ttctggtgag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1681 atcaaggcga acttcaagat ccgccacaac gtggaggacg gcagcgtgca gctcgccgac
     1741 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1801 ctgagctatc agtccgtgct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1861 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
     1921 accggaggga gcatgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1981 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat
     2041 gccaccaacg gcaagctgac cctgaagctc atctgcacca ccggcaagct gcccgtgccc
     2101 tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt gcttcgcccg ctaccccgac
     2161 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2221 accatcagct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc
     2281 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2341 ctggggcaca agctggagta caactttaac aaccccctga acatgaattt cgaactgtca
     2401 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaaact gcaggtcgac
     2461 gaacaaaaac tcatctcaga agaggatctg aatgctgtgg gccaggacac gcaggaggtc
     2521 atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat cctggccctg
     2581 gtggtgctca ccatcatctc ccttatcatc ctcatcatgc tttggcagaa gaagccacgt
     2641 tagaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3241 ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     3301 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3361 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3421 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     3481 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     3541 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     3601 ctctctgcgc gctcgctcgc tcactgaggc cgcccgggcw ttgcccgggc ggcsycmgkg
     3661 mktwkccssk scggcmwsrg kgrgrktkss staytggsys ctcttccgct tcctcgctca
     3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     5641 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5701 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     5761 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     5821 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     5881 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     5941 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6001 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6061 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctgcgcgctc gctcgctcac
     6181 tgaggccgcc cgssywwrrs ysrgsygatr ccgctcgccg cagccgaacg accgagcgca
     6241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6301 gttggccgat tcattaatgc agctggcrcg mcagstykcc cgactggaaa gcgggcagtg
     6361 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6421 tgcttccggc tcgtatgttg tgtggaattg tgagcssatw msmmwttcas rcwgsrmamc
     6481 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg
     6541 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
     6601 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
     6661 ttaatgatta acccgccatg ctac
//
