LOCUS       sequence-204604-        6134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Medium affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.GFAP.SF-Venus-iGluSnFR.A184V
FEATURES             Location/Qualifiers
     source          1..6134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     840..899
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1868..1889
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1992..2013)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2478..2507
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2511..2657
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2673..3261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2726..2746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3144..3155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3264..3280)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3265..3281)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3310..3431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3321..3340)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3375..3394
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3810..4398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3890..3909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4569..5360,5361..5429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5430..5534)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5560..6015)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5697..5718)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5909..5928
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   join(6087..6134,1..82)
                     /label=AAV2 ITR
ORIGIN
        1 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
       61 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacg
      121 tagccatgct ctaggaagat ccaacatatc ctggtgtgga gtaggggacg ctgctctgac
      181 agaggctcgg gggcctgagc tggctctgtg agctggggag gaggcagaca gccaggcctt
      241 tgtctgcaag cagacctggc agcattgggc tggccgcccc ccagggcctc ctcttcatgc
      301 ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag tgcctgcttc
      361 ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca gggggcttgc
      421 attgcacccc agcctgacag cctggcatct tgggataaaa gcagcacagc cccctagggg
      481 ctgcccttgc tgtgtggcgc caccggcggt ggagaacaag gctctattca gcctgtgccc
      541 aggaaagggg atcaggggat gcccaggcat ggacagtggg tggcaggggg ggagaggagg
      601 gctgtctgct tcccagaagt ccaaggacac aaatgggtga ggggagagct ctccccatag
      661 ctgggctgcg gcccaacccc accccctcag gctatgccag ggggtgttgc caggggcacc
      721 cgggcatcgc cagtctagcc cactccttca taaagccctc gcatcccagg agcgagcaga
      781 gccagagcag gttggagagg agacgcatca cctccgctgc tcgcggggat cccgccacca
      841 tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt tccactggtg
      901 acagatctgc cgcaggcagc acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg
      961 gtcaccgtga atcttcagtg cctttctctt attacgacaa tcagcaaaaa gtggtgggtt
     1021 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg
     1081 acttgcaggt aaaactgatt ccgattacct cacaaaaccg tattccactg ctgcaaaacg
     1141 gcactttcga ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg
     1201 ctttctctga cactattttc gtggtcggta cgcgcctgtt gaccaaaaag ggtggcgata
     1261 tcaaagattt tgccaacctg aaagacaaag ccgtagtcgt cacttccggc actacctctg
     1321 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca
     1381 aagatcacgg tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg
     1441 atgacgtgct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggaaatcg
     1501 tcggcaagcc gcagtctcag gaggcctacg gttgtatgtt gcgtaaagat gatccgcagt
     1561 tcaaaaagct gatggatgac accatcgctc aggtgcagac ctccggtgaa gcggaaaaat
     1621 ggtttgataa gtggttcaaa aatccaattc tggtgagcca caacgtctat atcaccgccg
     1681 acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacgtg gaggacggca
     1741 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1801 tgcccgacaa ccactacctg agctatcagt ccgtgctgag caaagacccc aacgagaagc
     1861 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1921 agctgtacaa gggcggtacc ggagggagca tgagcaaggg cgaggagctg ttcaccgggg
     1981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg
     2041 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagctcatc tgcaccaccg
     2101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc gtgcagtgct
     2161 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2221 gctacgtcca ggagcgcacc atcagcttca aggacgacgg cacctacaag acccgcgccg
     2281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctttaacaac cccctgaaca
     2401 tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac
     2461 tgaaactgca ggtcgacgaa caaaaactca tctcagaaga ggatctgaat gctgtgggcc
     2521 aggacacgca ggaggtcatc gtggtgccac actccttgcc ctttaaggtg gtggtgatct
     2581 cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc atcatgcttt
     2641 ggcagaagaa gccacgttag aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3241 ccctttgggc cgcctccccg catcgatacc gtcgacctcg acccgggcgg ccgcttcgag
     3301 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3361 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3421 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3481 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     3541 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     3601 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ctcgctgcgc
     3661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4681 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5521 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     5581 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5641 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5701 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5761 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     5821 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5881 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     5941 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6001 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6061 tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag gccgcccggg
     6121 caaagcccgg gcgt
//
