LOCUS       sequence-204609-        6615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Medium affinity yellow glutamate sensor ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.CAG.SF-Venus-iGluSnFR.A184V
FEATURES             Location/Qualifiers
     source          1..6615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   33..162
                     /label=AAV2 ITR
     promoter        473..750
                     /label=chicken beta-actin promoter
     primer_bind     1029..1051
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1113..1132
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1166..1175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     1172..1231
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2200..2221
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2324..2345)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2810..2839
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2843..2989
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    3005..3593
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3058..3078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3476..3487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3636..3757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3647..3666)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3701..3720
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3919..4048
                     /label=AAV2 ITR
     rep_origin      complement(4238..4826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4318..4337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4997..5788,5789..5857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5620..5639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5858..5962)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5988..6443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6125..6146)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6337..6356
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 twwcgsyrmg gkcctcttcg ctattacgcc agctgcgcgc tcgctcgctc actgaggccg
       61 cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
      121 cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc
      181 cgccatgcta cttatctacg tagccatgct ctaggaagag taccattgac gtcaataatg
      241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      361 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      421 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      481 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      541 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
      601 ccaggcgggg cggggcgggg cgrggggcgg ggcggggcga ggcggagagg tgcggcggca
      661 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      721 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      781 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      841 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      901 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
      961 tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     1021 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1081 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     1141 tcattttggc aaagaattgg atcccgccac catggagaca gacacactcc tgctatgggt
     1201 actgctgctc tgggttccag gttccactgg tgacagatct gccgcaggca gcacgctgga
     1261 caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tgcctttctc
     1321 ttattacgac aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt
     1381 tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga ttccgattac
     1441 ctcacaaaac cgtattccac tgctgcaaaa cggcactttc gattttgaat gtggttctac
     1501 caccaacaac gtcgaacgcc aaaaacaggc ggctttctct gacactattt tcgtggtcgg
     1561 tacgcgcctg ttgaccaaaa agggtggcga tatcaaagat tttgccaacc tgaaagacaa
     1621 agccgtagtc gtcacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga
     1681 gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgcaccct
     1741 ggaaagcggt cgtgccgttg cctttatgat ggatgacgtg ctgctggccg gtgaacgtgc
     1801 gaaagcgaag aaaccagaca actgggaaat cgtcggcaag ccgcagtctc aggaggccta
     1861 cggttgtatg ttgcgtaaag atgatccgca gttcaaaaag ctgatggatg acaccatcgc
     1921 tcaggtgcag acctccggtg aagcggaaaa atggtttgat aagtggttca aaaatccaat
     1981 tctggtgagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggcgaa
     2041 cttcaagatc cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca
     2101 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctatca
     2161 gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2221 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     2281 catgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2341 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
     2401 caagctgacc ctgaagctca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2461 cgtgaccacc ctgggctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     2521 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt
     2581 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2641 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2701 gctggagtac aactttaaca accccctgaa catgaatttc gaactgtcag acgaaatgaa
     2761 agcactgttc aaagaaccga atgacaaggc actgaaactg caggtcgacg aacaaaaact
     2821 catctcagaa gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc
     2881 acactccttg ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac
     2941 catcatctcc cttatcatcc tcatcatgct ttggcagaag aagccacgtt agaagcttat
     3001 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3061 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3601 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     3661 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3721 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3781 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     3841 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
     3901 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3961 ctcgctcgct cactgaggcc gsgcgrscwa aggtcgcccg acgcccgggc tttgcccggg
     4021 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgmwkmskcs ckcacygact
     4081 cgcygcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     6001 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6061 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6181 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6241 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6301 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6361 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6421 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     6481 gcgatcggtg cgggcctctt cgctattacg ccagctgmky sykygswyrm ycgtattacc
     6541 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6601 agcgaggaag cggaa
//
