LOCUS       sequence-20461-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..144)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(152..168)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..168)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(183..624)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(189..212)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    507..624
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        complement(648..666)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
       61 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      121 ttcagctttt ttgtacaaac ttgtgatggg ggatccacta gttctagaat ccggggtttt
      181 ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata cttttattac
      241 atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa gtggttatgc
      301 agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct gattaattac
      361 cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta atttgattcg
      421 ttcatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat aaccataaaa
      481 gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc
      541 aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccttcg gagggctgtc
      601 acccgctcgg cggcttctaa tccgtactag agctccagct tttgttccct ttagtgaggg
      661 ttaattg
//
