LOCUS       sequence-204611-        6842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Weak affinity glutamate sensor ** A newer version of this sensor is 
            available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.CAG.SF-iGluSnFR.S72A
FEATURES             Location/Qualifiers
     source          1..6842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   259..388
                     /label=AAV2 ITR
     promoter        699..976
                     /label=chicken beta-actin promoter
     primer_bind     1255..1277
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1339..1358
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1392..1401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     1398..1457
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2426..2447
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2550..2571)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             3036..3065
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3069..3215
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    3231..3819
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3284..3304)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3702..3713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3862..3983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3873..3892)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3927..3946
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4145..4274
                     /label=AAV2 ITR
     rep_origin      4456..4911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4543..4562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4753..4774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4937..5041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5042..5110,5111..5902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5260..5279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6073..6661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6562..6581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgnc
       61 gcgctcgctc gctcrmckgg rmmgcssgsr swragcgcrr cgcaattaat gtgagttagc
      121 tcrctcatya ggcacccyrg gcttwmcacc wcacccgccg cgcttaatgc gccgctacag
      181 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      241 tcttcgctat tacgccagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      721 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      781 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      841 gcggggcgrg gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      901 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      961 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1021 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1081 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1141 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1201 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1261 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     1321 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     1381 aattggatcc cgccaccatg gagacagaca cactcctgct atgggtactg ctgctctggg
     1441 ttccaggttc cactggtgac agatctgccg caggcagcac gctggacaaa atcgccaaaa
     1501 acggtgtgat tgtcgtcggt caccgtgaat cttcagtgcc tttctcttat tacgacaatc
     1561 agcaaaaagt ggtgggttac tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa
     1621 agaaactcaa caaaccggac ttgcaggtaa aactgattcc gattaccgca caaaaccgta
     1681 ttccactgct gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc aacaacgtcg
     1741 aacgccaaaa acaggcggct ttctctgaca ctattttcgt ggtcggtacg cgcctgttga
     1801 ccaaaaaggg tggcgatatc aaagattttg ccaacctgaa agacaaagcc gtagtcgtca
     1861 cttccggcac tacctctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata
     1921 tgcgcatcat cagcgccaaa gatcacggtg actctttccg caccctggaa agcggtcgtg
     1981 ccgttgcctt tatgatggat gacgtgctgc tggccggtga acgtgcgaaa gcgaagaaac
     2041 cagacaactg ggaaatcgtc ggcaagccgc agtctcagga ggcctacggt tgtatgttgc
     2101 gtaaagatga tccgcagttc aaaaagctga tggatgacac catcgctcag gtgcagacct
     2161 ccggtgaagc ggaaaaatgg tttgataagt ggttcaaaaa tccaattctg gtgagccaca
     2221 acgtctatat caccgccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc
     2281 acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2341 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gtgctgagca
     2401 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2461 tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg agcaagggcg
     2521 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2581 acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     2641 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2701 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2761 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag gacgacggca
     2821 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2881 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2941 ttaacaaccc cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag
     3001 aaccgaatga caaggcactg aaactgcagg tcgacgaaca aaaactcatc tcagaagagg
     3061 atctgaatgc tgtgggccag gacacgcagg aggtcatcgt ggtgccacac tccttgccct
     3121 ttaaggtggt ggtgatctca gccatcctgg ccctggtggt gctcaccatc atctccctta
     3181 tcatcctcat catgctttgg cagaagaagc cacgttagaa gcttatcgat aatcaacctc
     3241 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3301 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3361 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3421 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3481 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3541 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3601 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     3661 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3721 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3781 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacccgggc
     3841 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3901 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3961 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     4021 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     4081 ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     4141 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     4201 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     4261 gagcgagcgc gcagctgsmk taatrrmkms rmkkcmcksr ccgnyycact gaggccgggc
     4321 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4381 gcagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4441 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     4921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6841 ag
//
