LOCUS       sequence-204617-       10284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS
            specifically with Venus and DsRed lazers. Tet-ON-all-in-one.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pLT3REVIR_MARK3 1122
FEATURES             Location/Qualifiers
     source          1..10284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    619..852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1037..1081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1348..1465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1483..1861
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1491..1509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1527..1545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1563..1581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1599..1617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1635..1653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1671..1689
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1707..1725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1770..1794
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1885..2562
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2065..2085)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2477..2500
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           2746..2760
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        2939..3426
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2974..2997)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3359..3378
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3449..3451,3452..3454,3455..4168)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3494..3515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3755..3774)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4102..4123
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4181..4748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4198..4750
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4342..4359)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4569..4588
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4762..5379,5380..5385,5386..5421,5422..5424,5425..5460,
                     5461..5463,5464..5499,5500..5508)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5554..6142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5607..5627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6025..6036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6349..6582
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6654..6775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6691..6710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6745..6764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6815..6950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6970..6989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6971..6989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6999..7016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6999..7015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7008..7030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7157..7612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7244..7263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7454..7475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7638..7742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7743..7811,7812..8603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7961..7980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8774..9362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9263..9282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9516..9533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9650..9671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9686..9691,9692..9709,9710..9716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9724..9740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9729..9751
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9748..9764
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9748..9764
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9783..9803
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9785..9803
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9831..10057
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10058..10238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
       61 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      121 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      181 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      241 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
      301 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
      361 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
      421 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
      481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
      541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
      601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
      661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     1261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     1321 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
     1381 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1441 acaaattaca aaaattcaaa attttatcga tcctctagat tcgagtttac tccctatcag
     1501 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
     1561 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
     1621 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
     1681 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttag
     1741 gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1801 agcaattcca caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa
     1861 agtcgagctt gcgttggatc caccatggcc tcctccgaga acgtcatcac cgagttcatg
     1921 cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc
     2041 cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac
     2101 gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc
     2221 ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac
     2281 ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     2341 cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga cggcggccac
     2401 tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac
     2461 tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac catcgtggag
     2521 cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agcggccgcc tcgactaggg
     2581 ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg cctgcacatc
     2641 ttggaaacac ttgctgggat tacttcgact tcttaaccca acagaaggct cgagaaggta
     2701 tattgctgtt gacagtgagc gcaagaagca agatctaaat ttatagtgaa gccacagatg
     2761 tataaattta gatcttgctt cttttgccta ctgcctcgga cttcaagggg ctagaattcg
     2821 agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa
     2881 agctgaatta aaatggtata aattaaatca cttttttcaa ttgacgcgta attctaccgg
     2941 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     3001 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     3061 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     3121 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     3181 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     3241 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     3301 gggtccgggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     3361 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     3421 ctttcgacct gcagcccaag cttccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     3601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     3661 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3721 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3781 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3841 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3901 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     3961 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4021 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     4081 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4141 tctcggcatg gacgagctgt acaagtaaga attgttcaat tccgcccccc ccccctaacg
     4201 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4261 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4321 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4381 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4441 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4501 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4561 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4621 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4681 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4741 gatgataata tggccacaac catgagtaga ctggacaaga gcaaagtcat aaacggagct
     4801 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     4861 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     4921 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     4981 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     5041 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     5101 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     5161 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     5221 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     5281 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     5341 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     5401 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     5461 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaatc cggactcgac
     5521 ctgcagccaa gcttatcgaa acgcgtctgg aacaatcaac ctctggatta caaaatttgt
     5581 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5641 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5701 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5761 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5821 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5881 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5941 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6001 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6061 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6121 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     6181 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6241 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6301 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6361 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6421 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6481 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6541 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6601 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6781 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6961 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7141 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7381 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7441 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7501 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7561 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7741 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7801 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7861 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7921 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7981 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8041 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8101 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8161 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8221 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8281 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8341 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8401 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8461 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8521 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8581 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8641 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8701 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8881 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9541 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9601 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9661 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9721 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9781 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     9841 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9901 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9961 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
    10021 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
    10081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
    10261 aaagcgaaag ggaaaccaga ggag
//
