LOCUS       sequence-204618-        4114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MEF2C phosphomimetic mutant serine to aspartic acid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pINDUCER20 MEF2C S222D
FEATURES             Location/Qualifiers
     source          1..4114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    177..195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        270..308
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     305..329
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    438..462
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1905..1929)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1982..2008
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2094..3305
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3142..3161
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3364..3981,3982..3987,3988..4023,4024..4026,4027..4062,
                     4063..4065,4066..4101,4102..4110)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
       61 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      121 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      181 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      241 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
      301 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      361 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaactagtc cagtgtggtg
      421 gaattctgca gatatcaaca agtttgtaca aaaaagcagg ctgagccacc atggggagaa
      481 aaaagattca gattacgagg attatggatg aacgtaacag acaggtgaca tttacaaaga
      541 ggaaatttgg gttgatgaag aaggcttatg agctgagcgt gctgtgtgac tgtgagattg
      601 cgctgatcat cttcaacagc accaacaagc tgttccagta tgccagcacc gacatggaca
      661 aagtgcttct caagtacacg gagtacaacg agccgcatga gagccggaca aactcagaca
      721 tcgtggagac gttgagaaag aagggcctta atggctgtga cagcccagac cccgatgcgg
      781 acgattccgt aggtcacagc cctgagtctg aggacaagta caggaaaatt aacgaagata
      841 ttgatctaat gatcagcagg caaagattgt gtgctgttcc acctcccaac ttcgagatgc
      901 cagtctccat cccagtgtcc agccacaaca gtttggtgta cagcaaccct gtcagctcac
      961 tgggaaaccc caacctattg ccactggctc acccttctct gcagaggaat agtatgtctc
     1021 ctggtgtaac acatcgacct ccaagtgcag gtaacacagg tggtctgatg ggtggagacc
     1081 tcacgtctgg tgcaggcacc agtgcaggga acgggtatgg caatccccga aacgacccag
     1141 gtctgctggt ctcacctggt aacttgaaca agaatatgca agcaaaatct cctcccccaa
     1201 tgaatttagg aatgaataac cgtaaaccag atctccgagt tcttattcca ccaggcagca
     1261 agaatacgat gccatcagtg tctgaggatg tcgacctgct tttgaatcaa aggataaata
     1321 actcccagtc ggctcagtca ttggctaccc cagtggtttc cgtagcaact cctactttac
     1381 caggacaagg aatgggagga tatccatcag ccatttcaac aacatatggt accgagtact
     1441 ctctgagtag tgcagacctg tcatctctgt ctgggtttaa caccgccagc gctcttcacc
     1501 ttggttcagt aactggctgg caacagcaac acctacataa catgccacca tctgccctca
     1561 gtcagttggg agcttgcact agcactcatt tatctcagag ttcaaatctc tccctgcctt
     1621 ctactcaaag cctcaacatc aagtcagaac ctgtttctcc tcctagagac cgtaccacca
     1681 ccccttcgag atacccacaa cacacgcgcc acgaggcggg gagatctcct gttgacagct
     1741 tgagcagctg tagcagttcg tacgacggga gcgaccgaga ggatcaccgg aacgaattcc
     1801 actcccccat tggactcacc agaccttcgc cggacgaaag ggaaagtccc tcagtcaagc
     1861 gcatgcgact ttctgaagga tgggcaacat aaggatcccc cgggacccag ctttcttgta
     1921 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     1981 atacccatac gacgtcccag actacgctta gtaatgatta attaaactag aaattctacc
     2041 gggtagggga ggcgcttttc ccaaggcagt ctggagtaac ccacccaaga tctggcctcc
     2101 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     2161 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     2221 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     2281 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     2341 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     2401 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     2461 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     2521 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     2581 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     2641 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     2701 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     2761 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     2821 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     2881 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     2941 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     3001 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     3061 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     3121 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     3181 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     3241 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     3301 tagacgaagc ttggtaccga gctcggatct ccaccccgta ccggtcctgc agtcgaattc
     3361 accatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     3421 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     3481 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     3541 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     3601 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     3661 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     3721 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     3781 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     3841 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     3901 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     3961 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     4021 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     4081 gattttgacc ttgacatgct ccccgggtaa ctaa
//
