LOCUS       sequence-204619-        7207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MEF2C phosphomimetic mutant serine to aspartic acid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pINDUCER20 MEF2C S222D
FEATURES             Location/Qualifiers
     source          1..7207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             405..1199
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             405..1199
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(459..478)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1069..1088
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1376..1964
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1429..1449)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1847..1858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2039..2272
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2391..2409)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2477..2581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2582..2650,2651..3442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2800..2819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3613..4201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4102..4121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4355..4372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4612..4633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4648..4653,4654..4671,4672..4678)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4686..4702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4691..4713
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4710..4726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4710..4726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4776..4795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4987..5620
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5667..5792
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6289..6522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6707..6751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7044..7161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
       61 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
      121 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      181 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      241 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
      301 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
      361 tggttttcct ttgaaaaaca cgatgataag cttaccggtc caccatgatt gaacaagatg
      421 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
      481 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
      541 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
      601 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
      661 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
      721 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
      781 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
      841 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
      901 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
      961 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     1021 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     1081 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     1141 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
     1201 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     1261 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1321 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     1381 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1441 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1501 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1561 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1621 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1681 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1741 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     1801 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1861 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     1921 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     1981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2041 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     2101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     2281 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     2341 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     2401 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2461 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2521 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2581 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2641 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2701 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2761 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2821 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2881 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2941 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3001 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3061 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3121 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3181 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3241 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3301 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3361 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3421 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3481 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3541 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3601 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3661 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3721 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3781 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3841 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3961 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4021 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4081 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4141 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4201 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4261 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4321 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4381 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     4441 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4501 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     4561 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4621 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4681 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     4741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4801 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     4861 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     4921 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     4981 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     5041 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     5101 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     5161 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     5221 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     5281 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     5341 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     5401 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     5461 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5521 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5581 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     5641 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     5701 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5761 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     5821 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     5881 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     5941 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6001 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6061 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6121 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     6181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     6241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     6301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     6361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     6421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     6481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     6541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     6601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     6661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     6721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     6781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     6841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     6901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     6961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
     7021 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     7081 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7141 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact
     7201 aggatcc
//
