LOCUS       sequence-204626-       13447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to GFP-labelled RAB-11 expressed specifically in the 
            epidermis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, 
            Ewbank JJ, Pujol N
  TITLE     Microtubule plus-end dynamics link wound repair to the innate immune
            response.
  JOURNAL   Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047.
  PUBMED    31995031
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-29"; volume: "9. pii: 45047. doi"; pages: " 
            10.7554/eLife.45047"
COMMENT     Sequence Label: pNP159
FEATURES             Location/Qualifiers
     source          1..13447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(75..99)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1920..1942
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1980..1998)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2066..2170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2171..2239,2240..3031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2389..2408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3202..3790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3691..3710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(5278..5311)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(5311..5322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(6744..7067)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    8985..9009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9012..10037
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9012..10037
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(10038..10062)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10415..10448)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     10817..10834
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10818..10834
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11878..11906)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     12654..12674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    12715..12739
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggcggtgg cagtggtaca atcattcctt cgccagcgtc cgacccacca aagaagcagt
       61 gttgcatccc ataaaaccca ctttcttgta caaagtggac cattcgtaga attccaactg
      121 agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca
      181 tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc
      241 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
      301 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
      361 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
      421 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
      481 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
      541 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
      601 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tatgcgcgat gccgtatcac
      661 aactgagaaa aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa
      721 aaatcgagtt tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct
      781 cctgggcgtg gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa
      841 ttatttcata tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata
      901 caaacgaggc ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa
      961 ttagctattt cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct
     1021 aattagaaat gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa
     1081 tgatcccgca cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac
     1141 acaattcaag ttacattatt ttcaggaaac aatggcgaag agcaaagcag agattcaaaa
     1201 ggaggaaaga gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt
     1261 gagtaaccat gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa
     1321 cagtttcgaa aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc
     1381 ctaaacgagt gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta
     1441 tcctttcaaa tgcactctat caaacataga tcatatccat catatcttat ccagaattta
     1501 tatcatatcc aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta
     1561 gatcgacaac aaggcctcca cttcttccac gtgacgtcgt gactgggaaa accctggcgt
     1621 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1681 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     1741 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     1801 tgcaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1861 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1921 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     1981 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2161 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2221 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2281 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2341 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2401 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2461 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2521 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2581 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2641 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2701 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2761 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2821 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2881 gcgtgggtct cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt
     2941 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3001 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3061 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3121 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt
     3841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3901 tgagtgagct gataccagcg gataacaatt tcacagggcc ctgtcggatt atgggctctt
     3961 cttttatcct ctactttctt tctagaaaat gcaaatgttg agtcagaaat tgctgaatgg
     4021 agtacaactg caatgggttt agcttatgtt tctggaacaa ttactggtgg aattgtgatt
     4081 gaaaagtaag gttatatttc atttcaataa atgttaccac agtataacaa aaacaagtta
     4141 cattgttcag agtatgtctg aatgtattat tattattgta tttgtgcatc agtactgtag
     4201 ttgtactgaa gaagggttta aaataatctc aacgttttta agacgagtta gtgagaatac
     4261 ggtcgcagtt aggatgtttg gacatagttt tattagataa tcgtgaacta actcactata
     4321 aaaacccgct aactcattga ataagatagt tttaaacgtt caaaatttga agacaaaatt
     4381 gtatgaataa gtttacagag ttggtcgacg caagcttctc cttctcttca catttctcaa
     4441 taatttagct ctaattgcat tcgtattctt cgcaaaaata cgtattctga ttgacccgat
     4501 gaaatacgga tgcctagttg cattgatcat ctacggttat acttatgggt gaggtttttc
     4561 aaatatattt tgatttaatc gaataatgaa atttcagaac tggagttggt ccaatttcgt
     4621 ggtttatatc ctctgaactg gtacctcaaa aacatcgtag tattgctcaa tctgtggcat
     4681 attcaatgaa tactgtaatg gtggttatca gtacatttac agtattacct ctatattccg
     4741 ttattggtag ttatgctttc cttattctat attcaattcc ttcatttata agtatgctta
     4801 tattatttcg ctacttaccg gaaaccaaag gacgagagat tcatgatatt gtgaatgaac
     4861 ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc gaaattttac ttgatcgagc
     4921 caattgaact tttgttaaga cttacagaaa agtgagagat tttgttacaa aaaagtgaga
     4981 aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc cgaagtatca tataaaaatt
     5041 aaaaagattg tttgacctgg cggaactttt ttgcaaaaac tctgaatatc catggcacat
     5101 gcttcgtgca aaacaggatc caaatgaata tcaacaagag attcttgaag acgacgagcc
     5161 acttgctcag agcattgctt atctcgaatg agacccttgt taaaatcatt tttcaaacat
     5221 tatatgacat tgtcgaaatg tcctcctgat tccatgatgg tagcaaactc acttcgtata
     5281 acttcgtata gtatacctta tacgaagtta tctgccctcg attgtctatc ttccattctc
     5341 accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca accaatcttc
     5401 ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt actccttatc
     5461 tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc gtcggtaatg
     5521 taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata aaaggagacg
     5581 aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt acatttcttt
     5641 ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta aaacttgcgt
     5701 gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg accatgtcaa
     5761 ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg aatgtgttca
     5821 gagtgagtta aattatattt tgaattttaa ataattttta attttctagc aatctaccaa
     5881 tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc gttgtcaata
     5941 tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg ctccaccagg
     6001 tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg gagccaatgg
     6061 aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac catccggacc
     6121 aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg gtgttccagg
     6181 agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca cttgcccaca
     6241 aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac ttccaggacc
     6301 aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag gagagcaagg
     6361 atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga agggacgcga
     6421 cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc aaggaccaac
     6481 aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa ccgttggacc
     6541 agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg gagagactgg
     6601 accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca agtctccacc
     6661 atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg gtgttttcta
     6721 ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag tttcctcttt
     6781 ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt ttctcaacct
     6841 ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta catcagacag
     6901 aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga ctataaaaag
     6961 tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt tgacaaaaag
     7021 aaagaatagt gatagagaag aagggaatgc ttgaaaggat tttgcattta tcttaatcac
     7081 cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta atatagttca
     7141 tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg gaaactccag
     7201 cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa gccaagtaac
     7261 gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg aagattcctg
     7321 aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct tcaagagctc
     7381 gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga acacgacaga
     7441 acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc tcaacgagct
     7501 tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc gtttttgtac
     7561 gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg acgcgaatgc
     7621 gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag gcgaggtttc
     7681 ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg aagtcggttc
     7741 tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac ctttcgaagg
     7801 cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata cgtctcatta
     7861 cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga tggagcatat
     7921 tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct tggaggtaga
     7981 gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg ttaagcttgc
     8041 accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc tcggagaagg
     8101 cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt gtcgcgtcaa
     8161 ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt tcgaagtttt
     8221 ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct tatatacccg
     8281 cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt gaaaatactc
     8341 ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat tgtgtacact
     8401 acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct cgaatgttct
     8461 agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca acatttgctc
     8521 taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact atttccgtcc
     8581 agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta cacacgcgtc
     8641 tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt caacgtttat
     8701 acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt tcgatttttt
     8761 ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa aaatatgtta
     8821 ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt cagcataatt
     8881 tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt atgactgatc
     8941 tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt ttgtacaaaa
     9001 aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     9061 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     9121 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     9181 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     9241 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     9301 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     9361 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     9421 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     9481 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     9541 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     9601 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     9661 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     9721 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     9781 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     9841 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     9901 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     9961 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
    10021 cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta ctcttcaaca
    10081 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
    10141 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
    10201 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
    10261 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
    10321 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
    10381 tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagta taccttatac
    10441 gaagttatac tagtatatat ttttatgttt tcttttaaac cttcctccat atttctgaat
    10501 tgaatgttgt tgcatattgt gtgtccttat ttcatttcca tcatcctttt gtgtgtttcc
    10561 cccttccacc aattcccccc acgtcctaat gaaatgaaag cgagaagata aataaacttg
    10621 cacattgtgt tcagatgaga ggagcggaat acgaattggg agacggaaag agaaaaaaaa
    10681 atgtttagat ttggattgaa ttaatttttt agaaagcaag aaaaaaaaca caaagagaat
    10741 aaataaataa taggaaaaaa aacaaatatt cagtatttaa aatttgaaaa ttcaacgacg
    10801 ttggcgtcga tcatcctgta aaacgacggc cagtgccttg aagccatatg gtcttgctgg
    10861 aaactactgg gaaaagtttg gagaagtgat gattgatgtg gtactggcgc aagaggcagt
    10921 gcgagatctt ccgggagctg gacaggcttg ggtaattttt accgcttgct tggtggatca
    10981 gatgagagct ggatttgatg agaacagaaa aactgaccat gaagctcaaa tgaaaaaaaa
    11041 cacggttttg caccacgcta cgcagcagct attggaagac gttagttcga atgtttcaaa
    11101 aagttttaga gatgtttagt ttaagaatgt ggcatccacc tcagcaacaa ctgcaccgtc
    11161 tggaaatggt gagtgttcga gctccaacca actatccacg agctccaatc aaccgttggc
    11221 atgcccattt gcaagaatgt ctctgacgcc tgtgagttct aataggattt ttgcattcct
    11281 agttgataaa tgttcttatc caacgttttg caactgtaca catttcagaa ggacgctcgt
    11341 gtgatcgaaa gtctctccgg tagcggcggc agcggcagca gcggtaacgg agacatcagt
    11401 gacctctcgg gaacaatcta tttgtaatct cattccacga tttctcgcaa cacatccctt
    11461 atattctaca ttcctttttc gcatgtactc atctcttctc gtttggaatc ttttcttatc
    11521 tccgataatt gggttattgt gtcgataaag gctgacctat ttaacagata cactccacct
    11581 tcatccaccc ttccgttttc tacaatccat gttcttttca attcaattca tttatcacac
    11641 cctgttcttt tttttaattt atattttttg tcaaaactcg aaatattttt tctaccgctc
    11701 tgtgaaaaca aaacaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggattgg
    11761 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
    11821 gacccttggg gggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
    11881 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
    11941 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
    12001 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
    12061 aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac
    12121 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
    12181 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc
    12241 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
    12301 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
    12361 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
    12421 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
    12481 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
    12541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
    12601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
    12661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagcaagt
    12721 ttgtacaaaa aagcaggctt gatgggctct cgtgacgatg aatacgacta cttgttcaag
    12781 ggtaattttg tttttcattt ttcaattccc aaacgaaaaa ttgtttcagt tgttctgatt
    12841 ggagactcag gcgtcggaaa gtcgaatctc ctgtctcgtt tcacaagaaa tgagttcaac
    12901 ttggaatcaa aatcaacaat cggagtcgag tttgccacga gaagcatctc ggtgaggaac
    12961 ttcattagaa tacacttatg attcaaatta ttatttttgt aggtagaagg caagacagtg
    13021 aaggctcaaa tttgggatac tgctggacag gaacgttacc gtgccatcac atccgcttac
    13081 tatcgtgggg ctgtcggagc tctcctagtc tacgacatcg ctaagcatgt gacgtacgag
    13141 aatgttgagc gatggttgaa ggagcttcgt gatcacgccg atcagaacat tgtgattatg
    13201 ttggtcggaa acaagagcga cttgcgccat ttgcgtgcag ttccaacaga cgaggccaag
    13261 atctacgccg aaagaaatca attgtcgttt attggtaagt taaactaatc tattgcgttt
    13321 atatattttt ttttttcaga aacatctgcc ctcgacagca ccaacgttga agcagctttc
    13381 actaatatcc tgacggaaat ctacaaatca gtatccaaca agcatgtagg aactgacaga
    13441 caaggat
//
