LOCUS       sequence-204627-       11064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - 
            EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A -
            MTS - tFNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-245
FEATURES             Location/Qualifiers
     source          1..11064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1264..1338
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1360..1369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..1398
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2137..2193
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2194..2268
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2296..2319
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2760..2769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3058..3114
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3115..3189
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3211..3220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3220..3261
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3571..3627
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3628..3702
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3733..3750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4943..4963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4948..5277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5128..5263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5287..5886
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5287..5306)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5783..5803
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6179..6412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6563..6580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6569..6793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      6836..6845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6889..6993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6994..7062,7063..7854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7212..7231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8025..8613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8514..8533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             8772..9405
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9452..9577
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10070..10303
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10488..10532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10819..10936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgccgcc
     1261 accatgtccg tgctgacccc actcctgctt aggggcctta ccggctctgc caggcgcctc
     1321 ccggtcccac gggcaaagat ccactcattg ttcgaagccg ccaccatgga acagaagctg
     1381 atctccgaag aggacctgat gggcacagcc accatgacaa ccagtttggc tacaaagttg
     1441 agagagggta ctaaaaaggc tcacaccatg gcagagaacg tggggttcgt gcggtgtttt
     1501 ctgaagggga cagttgagaa aagctcttat aggaaactgg tcgcgtcctt gtaccatgtc
     1561 tattccgcga tggagcaaga gatggagaga ttgaaggatc acccaatcgt tgggaagatc
     1621 tattttcctg agctgaatcg gaagagtagt ctggagaggg atttgaccta ttactttggt
     1681 agcaattggc gcgaagagat cccaccctct ccagccactc aggcttacgt ggcccgaatc
     1741 catgaggtgg caaacactgc ccccgaactg ctggtagcac attcatatac tcgatatctt
     1801 ggggatctct ccggaggtca gattcttaaa ggcatcgctg agcgagcaat gaatctgcaa
     1861 gatggagagg gcactgcttt ctatcggttt gagagcatct ctgacgagaa agcctttaaa
     1921 cagctgtatc gacagcggct cgacgaactg cctgtcgacg aggccaccgc agaccgcatc
     1981 gtcgacgagg ctaatgctgc tttcggaatg aacatgaaga tctttcagga gctggaaggg
     2041 aatctgatcc gggctatcgg tcaactgctc tttaacactc tgaccaggcg gaagcagagg
     2101 gggtctactg agcttgctac agccgatgga tctggggcca caaatttttc cctgctgaaa
     2161 caggctgggg acgtggagga aaatccaggc cccatgagcg ttctcacacc tttgctgctc
     2221 cgggggctca caggcagtgc tcgacgactg ccagtcccga gggcgaagat tcactcactg
     2281 gctagcggat caatggatta caaggatgac gacgataagt ctagcatggg gactgcaact
     2341 atgtctctga ggcaacatca acatcctctc atccagagac tcgcagatag aattgaggca
     2401 atttggcagg cctttttccc actggctcca tacgcactgc cggaggactt gggctacgtg
     2461 gaagggaagc ttgaaggcga gaggttgact attgaaaatc actgttatca ggccccgcct
     2521 tttagaaaac tgcatttgga gttggcgagg gtcggtgagt ctctggatat tttgcactgt
     2581 gtcatgtttc ccgaacctcg gtacgacctt cccatgttcg gctgcgatct ggtgggaggc
     2641 cggggccaaa tctctgccgc aattgttgac ctctcaccag tgaccggaca gttgccagct
     2701 gcctacacct gtgctttgaa cgcactgcct aagctgacat ttagacagcc ccgagagctg
     2761 ccaccatggg gacacatttt ttcacccttc tgcattttta ttaggcccca gggcgaggct
     2821 gaggagcagc agtttttgga tagaatcggt gaatatctta ctctgcactg ccagttgtcc
     2881 caacaggctg tgccaaccga ccaccctcaa gcagtcattg ctggccagcg gcagtattgt
     2941 cagcagcagc agcaaaacga taagacaagg agggtactgg agaaggcatt tggtgtaccc
     3001 tgggccgagc ggtatatgac tactgttctg ttcgacgttc cacccgtggg aagcggagct
     3061 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatgagc
     3121 gtgctgacgc ccttgctgct tagaggactg accggaagcg cacgccgcct gcccgtgcct
     3181 agggcgaaga tacatagcct gttcgaagcc gccaccatgg gtaagcccat tcccaacccg
     3241 ctgttgggcc tcgatagcac agttaacatg gctacctaca aagtgacact ggttcggcct
     3301 gatggtagcg agacaacaat tgacgtgcct gaggacgaat acatactcga cgttgcggaa
     3361 gagcaagggc tggatctgcc atttagttgc cgagcgggcg cttgcagcac ttgtgccggg
     3421 aagttgctcg aaggggaagt ggatcagtct gatcagtcct ttctggatga cgatcagatc
     3481 gaaaaagggt tcgtgctcac ctgtgtcgct tatccaagga gcgattgtaa gattctcact
     3541 aatcaggagg aggagcttta cggctcaggc gcgacaaatt tttctctcct caaacaggct
     3601 ggggacgtgg aggagaaccc tggccctatg tccgttctta ccccactgtt gttgcgggga
     3661 ctcactggat cggctagacg actccccgtc ccaagggcaa agatccattc tctggaattc
     3721 gccgccacca tgcaccacca ccatcaccac gttaacatgt acaatgcaac caactccagg
     3781 agcagaatgt ttagatatga agtagtagga ctgaggcaga ctgccgaaac cgaaaagacc
     3841 aactatgcca tacgaaatag cggaagccag ttttttaacg taccctatga taggatgaat
     3901 cagttcatgc agcagataac acgctggggc ggaaagatcg tatccattca gcccttgaac
     3961 ggcacagtgg ctcccctggc tgccaccacg gaacccgctg caaataatgg cgctgcgccc
     4021 gtcaaagaga aaaaggttga tataccagtt aacatctatc gcccgaacaa cccctgtatt
     4081 ggaaaagtaa tatccaacga ggaactggtg agggagggcg gtgagggaac cgtgaaacac
     4141 attatttttg atatctctgg aaccgagctc agatatttgg agggccagtc tatcgggatc
     4201 attccggctg ggaccgacgc aaatggtaaa ccacataaac tccggctgta ttctatcgcc
     4261 tcaacgaggc acggggactt tcaggatgac aagaccgtct cactgtgcgt tagacgcctc
     4321 gaatataaag ataaagagac tggcgagacc atttatggcg tgtgctcctc ctacctgaat
     4381 cagctgcagc ctggagacga agtgaagatt actggtcccg ttgggaagga aatgctgttg
     4441 agtgacgacc ctgaagccac catcataatg cttgctaccg gcaccggaat tgcccccttt
     4501 cgcgctttct tgtggcggat gttcaaagaa aacaacccag attatcagtt caaaggactt
     4561 gcctggctgt tttttggcgt cgcctacacc gctaacatcc tgtataaaga tgagctggag
     4621 gccatccagg cccagtatcc tgatcacttt cggcttacat acgcaatttc acgagagcag
     4681 aagactccgg acggtgggaa aatgtacatc caggggcgaa tcgccgaaca tgctgacgag
     4741 atttggcagt tgcttcagaa aaaaaacaca cacgtctata tgtgcggcct gaggggcatg
     4801 gaacctggga tagatgaagc aatgacagca gccgccgcca agaatggcgc agattggcag
     4861 gaatttctta aaggaacatt gaagaaagag ggacggtggc acgtggaaac atattaataa
     4921 ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5281 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5341 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5401 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5461 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5521 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5581 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5641 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5701 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5761 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5821 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5881 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     5941 tcgataaaat aaaagatttt atttagtctc cagaaaaagg gggcggccgc tcgagaccta
     6001 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6061 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6181 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6421 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6481 aggccttgac attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa
     6541 agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6661 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6721 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6781 gtgggctcta tggcttctga ggcggaaaga accagctggg ggcgcgcccc tcgaggccgc
     6841 catggtcata gctgtttgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcacgagaca ataaccctga
     6961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7201 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8641 ttctggcctt ttgcacggac cgccactgcc aattacctgt ggtttcattt actctaaacc
     8701 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     8761 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     8821 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8881 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8941 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     9001 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     9061 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     9121 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     9181 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     9241 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9301 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9361 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9421 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9481 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9541 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9601 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9661 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9721 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9781 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9841 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9901 caccgcacag caaccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9961 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10021 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10081 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10141 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10201 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    10261 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10321 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10381 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10441 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10501 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10561 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10621 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10681 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    10741 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccacaaat
    10801 ggcagtattc atccacaatt ttaaaagaaa gggggggatt ggggggtaca gtgcagggga
    10861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10921 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggatcgataa
    10981 gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc
    11041 taggtcttga aaggagtggg aatt
//
