LOCUS       sequence-204629-        9687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of CRISPR/dCas9 fused with SET domain of mouse 
            MLL3 gene, driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Yan J, Chen SA, Local A, Liu T, Qiu Y, Dorighi KM, Preissl S, Rivera
            CM, Wang C, Ye Z, Ge K, Hu M, Wysocka J, Ren B
  TITLE     Histone H3 lysine 4 monomethylation modulates long-range chromatin 
            interactions at enhancers.
  JOURNAL   Cell Res. 2018 Jan 9. pii: cr20181. doi: 10.1038/cr.2018.1.
  PUBMED    29313530
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2018 Jan 9. pii: cr20181. doi: 10.1038/cr.2018.1."
COMMENT     Sequence Label: CBh-dCas9-Mll3SET
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        338..623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        625..902
                     /label=chicken beta-actin promoter
     intron          903..1131
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1143..1152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1152..1217
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1221..1247,1248..1250,1251..3734,3735..3740,3741..3806,
                     3807..3809,3810..5324)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1221..1247,1248..1250,1251..3737,3738..3740,
                     3741..5324)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1221..5324
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    5325..5349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5917..5941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5955..5975
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6028..6650
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6227..6246)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6674..6694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6679..7008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6859..6994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7046..7065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7047..7065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7079..7095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7079..7095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7092..7114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7103..7119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7127..7133,7134..7151,7152..7157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7172..7193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7310..7327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7481..8069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7561..7580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8240..9031,9032..9100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8863..8882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9101..9205)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9232..9660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9319..9338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9529..9550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagtcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
      421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      541 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      601 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      661 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      721 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      781 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      841 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      901 cgggagtcgc tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      961 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     1021 cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg
     1081 gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg
     1141 gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat atcgattaca
     1201 aggatgacga tgacaagctc atggacaaga agtactccat tgggctcgct atcggcacaa
     1261 acagcgtcgg ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag
     1321 ttctgggcaa taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg
     1381 actccgggga gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc
     1441 gcagaaagaa tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg
     1501 atgactcttt cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg
     1561 agcgccaccc aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa
     1621 ccatatatca tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga
     1681 tctatctcgc gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc
     1741 tgaacccaga caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc
     1801 agcttttcga agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg
     1861 ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga
     1921 agaacggcct gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat
     1981 ctaacttcga cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg
     2041 atctcgacaa tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa
     2101 agaacctgtc agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca
     2161 aagctccgct gagcgctagt atgatcaagc gctatgatga gcaccaccaa gacttgactt
     2221 tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta caaggaaatt ttcttcgatc
     2281 agtctaaaaa tggctacgcc ggatacattg acggcggagc aagccaggag gaattttaca
     2341 aatttattaa gcccatcttg gaaaaaatgg acggcaccga ggagctgctg gtaaagctta
     2401 acagagaaga tctgttgcgc aaacagcgca ctttcgacaa tggaagcatc ccccaccaga
     2461 ttcacctggg cgaactgcac gctatcctca ggcggcaaga ggatttctac ccctttttga
     2521 aagataacag ggaaaagatt gagaaaatcc tcacatttcg gataccctac tatgtaggcc
     2581 ccctcgcccg gggaaattcc agattcgcgt ggatgactcg caaatcagaa gagaccatca
     2641 ctccctggaa cttcgaggaa gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa
     2701 ggatgactaa ctttgataaa aatctgccta acgaaaaggt gcttcctaaa cactctctgc
     2761 tgtacgagta cttcacagtt tataacgagc tcaccaaggt caaatacgtc acagaaggga
     2821 tgagaaagcc agcattcctg tctggagagc agaagaaagc tatcgtggac ctcctcttca
     2881 agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat
     2941 gtttcgactc tgttgaaatc agcggagtgg aggatcgctt caacgcatcc ctgggaacgt
     3001 atcacgatct cctgaaaatc attaaagaca aggacttcct ggacaatgag gagaacgagg
     3061 acattcttga ggacattgtc ctcaccctta cgttgtttga agatagggag atgattgaag
     3121 aacgcttgaa aacttacgct catctcttcg acgacaaagt catgaaacag ctcaagaggc
     3181 gccgatatac aggatggggg cggctgtcaa gaaaactgat caatgggatc cgagacaagc
     3241 agagtggaaa gacaatcctg gattttctta agtccgatgg atttgccaac aggaacttca
     3301 tgcagttgat ccatgatgac tctctcacct ttaaggagga catccagaaa gcacaagttt
     3361 ctggccaggg ggacagtctt cacgagcaca tcgctaatct tgcaggtagc ccagctatca
     3421 aaaagggaat actgcagacc gttaaggtcg tggatgaact cgtcaaagta atgggaaggc
     3481 ataagcccga gaatatcgtt atcgagatgg cccgagagaa ccaaactacc cagaagggac
     3541 agaagaacag tagggaaagg atgaagagga ttgaagaggg tataaaagaa ctggggtccc
     3601 aaatccttaa ggaacaccca gttgaaaaca cccagcttca gaatgagaag ctctacctgt
     3661 actacctgca gaacggcagg gacatgtacg tggatcagga actggacatc aatcggctct
     3721 ccgactacga cgtggatgcc atcgtgcccc agtcttttct caaagatgat tctattgata
     3781 ataaagtgtt gacaagatcc gataaaaata gagggaagag tgataacgtc ccctcagaag
     3841 aagttgtcaa gaaaatgaaa aattattggc ggcagctgct gaacgccaaa ctgatcacac
     3901 aacggaagtt cgataatctg actaaggctg aacgaggtgg cctgtctgag ttggataaag
     3961 ccggcttcat caaaaggcag cttgttgaga cacgccagat caccaagcac gtggcccaaa
     4021 ttctcgattc acgcatgaac accaagtacg atgaaaatga caaactgatt cgagaggtga
     4081 aagttattac tctgaagtct aagctggtct cagatttcag aaaggacttt cagttttata
     4141 aggtgagaga gatcaacaat taccaccatg cgcatgatgc ctacctgaat gcagtggtag
     4201 gcactgcact tatcaaaaaa tatcccaagc ttgaatctga atttgtttac ggagactata
     4261 aagtgtacga tgttaggaaa atgatcgcaa agtctgagca ggaaataggc aaggccaccg
     4321 ctaagtactt cttttacagc aatattatga attttttcaa gaccgagatt acactggcca
     4381 atggagagat tcggaagcga ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt
     4441 gggacaaggg tagggatttc gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca
     4501 tcgttaaaaa gaccgaagta cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa
     4561 ggaacagcga caagctgatc gcacgcaaaa aagattggga ccccaagaaa tacggcggat
     4621 tcgattctcc tacagtcgct tacagtgtac tggttgtggc caaagtggag aaagggaagt
     4681 ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac aatcatggag cgatcaagct
     4741 tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata taaagaggtc aaaaaagacc
     4801 tcatcattaa gcttcccaag tactctctct ttgagcttga aaacggccgg aaacgaatgc
     4861 tcgctagtgc gggcgagctg cagaaaggta acgagctggc actgccctct aaatacgtta
     4921 atttcttgta tctggccagc cactatgaaa agctcaaagg gtctcccgaa gataatgagc
     4981 agaagcagct gttcgtggaa caacacaaac actaccttga tgagatcatc gagcaaataa
     5041 gcgaattctc caaaagagtg atcctcgccg acgctaacct cgataaggtg ctttctgctt
     5101 acaataagca cagggataag cccatcaggg agcaggcaga aaacattatc cacttgttta
     5161 ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt cgacaccacc atagacagaa
     5221 agcggtacac ctctacaaag gaggtcctgg acgccacact gattcatcag tcaattacgg
     5281 ggctctatga aacaagaatc gacctctctc agctcggtgg agacacaagt ttgtacaaaa
     5341 aagcaggctt catgaggcct cacaccttga atagcaccag cacttcaaag tcatttcaga
     5401 gcacagtcac tggagagctg aatgcaccct acagtaagca gtttgtccac tccaagtcat
     5461 cacagtaccg gagaatgaag actgaatgga aatctaatgt gtatctggcc cgatctcgga
     5521 tccagggact gggcctgtat gctgctagag acattgaaaa acacactatg gtcatcgagt
     5581 acattggaac aattattcga aatgaggttg caaaccggaa ggagaagctt tatgagtctc
     5641 agaaccgggg tgtgtacatg ttccgcatgg acaatgacca cgtgattgac gccacactca
     5701 caggagggcc tgcaagatac atcaaccact cctgtgcccc taactgtgtg gctgaagtcg
     5761 tgacctttga gagaggacac aagattatca tcagctccaa ccggaggata cagaaaggag
     5821 aagagctctg ctatgattat aagtttgact ttgaagatga ccagcacaag attccgtgtc
     5881 actgtggagc ggtgaactgc cggaagtgga tgaacaaccc agctttcttg tacaaagtgg
     5941 ttagcagggc tgaccccaag aagaagagga aggtgtaaaa gggttggatc cctaccggat
     6001 cggtaccagt cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct
     6061 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     6121 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     6181 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     6241 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     6301 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     6361 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     6421 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     6481 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     6541 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     6601 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     6661 ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     6721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag
     7021 gaactgaaaa accagaaagt taattcccta tagtgagtcg tattaaattc gtaatcatgt
     7081 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7141 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7201 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7921 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8101 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8161 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8221 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8281 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8341 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8401 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8461 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8521 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8581 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8641 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8701 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8761 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8821 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8881 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8941 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9001 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9061 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9181 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     9241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9421 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9541 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9661 ttaacaaaat attaacgctt acaattt
//
