LOCUS       sequence-204632-        8634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Scot Wolfe
  TITLE     Inducible Cas9-DNA-binding-domain fusions for precise genome editing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSD_SpCas9MT3-FKBP_SL
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..43
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     25..42
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..4262
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(4311..4313,4314..4634)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     polyA_signal    4676..4810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4700..4719)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4754..4773
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4923..4943)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4923..4941)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4962..4978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4962..4978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4975..4997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4986..5002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5010..5016,5017..5034,5035..5040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5055..5076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5193..5210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5364..5952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5444..5463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6123..6914,6915..6983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6746..6765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6984..7088)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7114..7569)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7251..7272)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7463..7482
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7640..8624
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag ctacttgttc
       61 tttttgcagg atcccatcga ttcgaattca aggatcaaca agtttgtaca aaaaagcagg
      121 ctggcgccac catggccggg actagtgggc gtcccgggtc cgacaagaag tacagcatcg
      181 gcctggatat cggtaccaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
      241 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
      301 ttggagccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
      361 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
      421 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
      481 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
      541 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gctggtagac agtactgata
      601 aggctgactt gcggttgatc tatctcgcgc tggcgcacat gatcaaattt cggggacact
      661 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
      721 tggttcagac ttacaatcag cttttcgagg agaacccgat caacgcatcc ggcgttgacg
      781 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
      841 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
      901 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctg caactgagca
      961 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag
     1021 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag
     1081 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc
     1141 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca
     1201 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa
     1261 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg
     1321 agctgctggt aaagctgaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg
     1381 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg
     1441 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga
     1501 taccctacta tgtaggcccc ctcgctcggg gaaattccag attcgcgtgg atgactcgca
     1561 aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg
     1621 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc
     1681 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca
     1741 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta
     1801 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact
     1861 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca
     1921 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg
     1981 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag
     2041 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca
     2101 tgaaacagct caagagacgc cgatatacag gatgggggcg gctgtcaaga aaactgatca
     2161 atggcatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat
     2221 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca
     2281 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg
     2341 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg
     2401 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc
     2461 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta
     2521 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga
     2581 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac
     2641 tggacatcaa ccggttgtcc gactacgacg tggatcatat cgtgccccaa agctttctca
     2701 aagatgattc tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg
     2761 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga
     2821 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc
     2881 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca
     2941 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca
     3001 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa
     3061 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct
     3121 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctg gaatctgaat
     3181 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg
     3241 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga
     3301 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag
     3361 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgc aaggtcctgt
     3421 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg
     3481 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc
     3541 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca
     3601 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa
     3661 tcatggagcg atccagcttc gagaaaaacc ccatcgactt tctcgaagcg aaaggatata
     3721 aagaggtcaa aaaagacctc atcattaagc tgcccaagta ctctctcttt gagcttgaaa
     3781 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac
     3841 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt
     3901 ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg
     3961 agatcatcga gcaaataagc gagttctcca aaagagtgat cctcgccgac gctaacctcg
     4021 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa
     4081 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg
     4141 acaccaccat agacagaaag aagtacacct ctacaaagga ggtcctggac gccacactga
     4201 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag
     4261 acggcaccgg cgggcctagg gcgggctcca gggggagtgg gagctccgga atgggagtgc
     4321 aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg
     4381 tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctct cgggacagaa
     4441 acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg
     4501 ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg
     4561 gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc
     4621 ttctaaaact ggaactcgag taataatcta gaactatagt gagtcgtatt acgtagatcc
     4681 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4741 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4801 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4861 ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
     4921 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     4981 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
     5041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7081 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     7141 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7201 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     7261 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     7321 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     7381 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     7441 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     7501 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     7561 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     7621 cctcttcgct attacgccag tcgaccgcca attcaatatg gcgtatatgg actcatgcca
     7681 attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca
     7741 attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca
     7801 attcaatatg gcggacctgg cactgtgcca actggggagg ggtctacttg gcacggtgcc
     7861 aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc
     7921 aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg
     7981 ccctatgcca atatggctat tggccaggtt caatactatg tattggccct atgccatata
     8041 gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat
     8101 ataccatata tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt
     8161 ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc
     8221 ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca
     8281 ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc
     8341 acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat
     8401 taatagtaac ttggcaagta cattactatt ggaagtacgc cagggtacat tggcagtact
     8461 cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac
     8521 gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc
     8581 gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaaccgccat tctg
//
