LOCUS       sequence-20464-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..163)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(171..187)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(171..187)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(193..456)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(276..299)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 taaagtgtaa agcctggggg gtggcctaat gcggccgcca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtgatgggg gatccactag
      181 ttctagaatc cgtcgaaact aagttctggt gttttaaaac taaaaaaaag actaactata
      241 aaagtagaat ttaagaagtt taagaaatag atttacagaa ttacaatcaa tacctaccgt
      301 ctttatatac ttattagtca agtaggggaa taatttcagg gaactggttt caaccttttt
      361 tttcagcttt ttccaaatca gagagagcag aaggtaatag aaggtgtaag aaaatgagat
      421 agatacatgc gtgggtcaat tgccttgtgt catcatttac tccaggcagg ttgcatcact
      481 ccattgaggt tgtgcccgtt ttttgcctgt ttgtgcccct gttctctgta gttgcgctaa
      541 gagaatggac ctatgaactg atggttggtg aagaaaacaa tattttggtg ctgggattct
      601 ttttttttct ggatgccagc ttaaaaagcg ggctccatta tatttattgg atgccaggaa
      661 taaactgttc acccagacac ctacgatgtt atatatt
//
