LOCUS       sequence-204650-        4372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Plcb2
FEATURES             Location/Qualifiers
     source          1..4372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1514..1532)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1515..1532)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1550..1566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1550..1566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1563..1585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1574..1590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1598..1604,1605..1622,1623..1628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1643..1664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1781..1798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2032..2051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2711..3502,3503..3571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3334..3353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3572..3676)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3754..4209)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3891..3912)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4103..4122
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4335..4357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4349..4366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4350..4366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc
       61 ggccgcggga attcgattat cctgtctgga aagaggaacc ctttatcttt gagaagatct
      121 tgatgcctga gctggcctcc ctcaggatag ctgtgatgga agaaggcagc aaatttcttg
      181 gacaccgcat catccccatc aatgccttac attctggata ccaccatctg tgcctgcgca
      241 gtgagagcaa catggccctt accatgccag ctctctttgt cttcctggag atgaaggact
      301 acatacctga cacctgggca gatcttacag tagcccttgc caaccccatc aagtacttca
      361 atgcccagga taagaagtcc gtgaagctca aaggagtgac aggaagtctg cccgagaaac
      421 tcttctctgg gacacctgtt gccagtcagt ccaatggggc gccagtctcg gcaggcaatg
      481 ggtcaacagc gcctgggacc aaggccacgg gggaagaagc tacgaaagaa gtgacagagc
      541 cacagaccgc cagcttggaa gagttgcggg aactgaaggg cgtggtgaaa ctgcagcgaa
      601 gacatgagaa ggagcttcga gagttggagc gccgtggagc ccggcgctgg gaggaactgc
      661 tgcagcgtgg cgccgcacag ctggcggagc tccagaccca ggcagcaggc tgcaagctcc
      721 gcccaggcaa gggctcccgc aagaagagga ccctgccctg tgaggagacc gtcgtggcgc
      781 ctagcgagcc ccacgaccgg gcggacccgc gcgtgcagga gctgaaggac aggctggagc
      841 aggagctgca gcagcaaggc gaggagcagt accgctccgt cctcaagcgc aaggagcagc
      901 acgtgaccga gcaaatcgcc aagatgatgg agctggccag agagaaacag gctgctgaac
      961 tcaagacctt caaggagacc tcagaaaccg acaccaaaga aatgaagaaa aaactggagg
     1021 ccaagaggct ggaacggatc caagccatga ccaaggtcac cacagacaag gtggcccagg
     1081 agaggctcaa gagagaaatt aacaactccc acatccagga agtggtccag gctgtcaagc
     1141 agatgacaga gaccctggag cgtcaccagg agaagctgga agagaggcag acagcctgcc
     1201 tggagcagat ccaggcaatg gaaaagcagt tccaggagaa ggcgctggca gagtatgagg
     1261 ccaagatgaa gggcctggag gctgaagtaa aggagtcggt gcgggcctac ttcaaggact
     1321 gcttccccac cgaagcagaa gacaagcctg agaggtcctg tgaggcctct gaggagtcgt
     1381 gtccacagga accacttgtg agcaaggcag acactcagga gagccgcctc tgaaatcact
     1441 agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc
     1501 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
     1561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg tgaaataccg
     3721 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
     3781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4201 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4261 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     4321 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tg
//
