LOCUS       sequence-204653-        4068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Nqo1
FEATURES             Location/Qualifiers
     source          1..4068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      256..265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1166..1175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1170..1179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1209..1228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1210..1228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1235..1252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1235..1251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1244..1266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1392..1847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1479..1498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1689..1710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1925..2029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2030..2098,2099..2890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2248..2267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3061..3649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3550..3569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3803..3820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3937..3958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3973..3978,3979..3996,3997..4003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4011..4027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4016..4038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4035..4051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4035..4051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgacc tgcaggcggc cgcgaattca ctagtgatta agccttcgct cagcccatac
      121 cccaaggctc agctcttact agcctagcct gtagccagcc ctaaggatct ctccgaagag
      181 ctttagggtc gtcttggcaa ccagctgctc agccaatcag cgttcggtat tacgatcctc
      241 cctcaacatc tggagccatg gcggcgagaa gagccctgat tgtactggcc cattcagaga
      301 agacatcatt caactacgcc atgaaggagg ctgctgtaga ggctctgaag aagagaggat
      361 gggaggtact cgaatctgac ctctatgcta tgaacttcaa ccccatcatt tccagaaatg
      421 acatcacagg tgagctgaag gactcgaaga actttcagta tccttccgag tcatctctag
      481 catataagga aggacgcctg agcccagata ttgtggccga acacaagaag ctggaagctg
      541 cagacctggt gatatttcag ttcccattgc agtggtttgg ggtgccagcc attctgaaag
      601 gctggtttga gagagtgctc gtagcaggat ttgcctacac atatgctgcc atgtacgaca
      661 acggtccttt ccagaataag aagaccttgc tttctatcac cactgggggt agcggctcca
      721 tgtactctct tcagggtgtc cacggggaca tgaacgtcat tctctggccg attcagagtg
      781 gcatcctgcg tttctgtggc ttccaggcct tagaacctca actggtttac agcattggcc
      841 acactccacc agatgcccgc atgcagatcc tggaaggatg gaagaaacgt ctggaaaccg
      901 tctgggagga gaccccactc tattttgctc caagcagcct gtttgaccta aactttcagg
      961 caggattctt aatgaaaaag gaagttcaag aggagcagaa gaagaacaag tttggcctct
     1021 ctgtgggcca tcacctgggc aagtccattc cagctgacaa ccagatcaaa gctagaaaat
     1081 aaggattttt ttcctaacat atagttagac gcagctttct ttttccccag cttgtctgac
     1141 ttgcttgctt aatcgaattc ccgcggccgc catggcggcc gggagcatgc gacgtcgggc
     1201 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     1261 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1321 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1381 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1441 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1741 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1801 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     1861 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     1921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3721 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3781 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3841 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3901 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3961 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4021 ataacaattt cacacaggaa acagctatga ccatgattac gccaagct
//
