LOCUS       sequence-204659-        3881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Vmn1r168
FEATURES             Location/Qualifiers
     source          1..3881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      979..988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1022..1041)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1023..1041)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1048..1065)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1048..1064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1057..1079)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1205..1660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1292..1311)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1502..1523
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1738..1842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1843..1911,1912..2703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2061..2080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2874..3462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3363..3382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3616..3633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3750..3771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3786..3791,3792..3809,3810..3816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3824..3840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3829..3851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3848..3864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3848..3864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgacc tgcaggcggc cgcgaattca ctagtgatta tgtcttctca gaatggagcc
      121 atgaaaacca ctgaggaagt ggcacttcag attgtgttgc tttgtcagtt tgggataggg
      181 actatggcta acattcttct gtttgtccat aatttctctc cacttttgac tggatgtcag
      241 ctgagaccca aagaagtgat ttgtgctaac atggctgtgt ccagcatctt aattctattc
      301 atgactgtgt ttccaaacaa tgtgtttgtt gctcaaagga atttactgaa tgaccttcaa
      361 tgtaaacttg agtactttgt atacatggta gcaagaagca caaacttgtg ttccacctgt
      421 gtcttgagca cctatcagtt tgtcactctt gttcctggta atttcagtga gctgatggtc
      481 agaggaagaa gccccaaggt cataagctat tcctgttgta gttgttggtt gtttggtatc
      541 ttatataatg tctacattcc aatgaaagtc agtggtccac agaacagaca taataaaacc
      601 agcactaaaa gtaagtgggt ctgttttaca tctggcttca gtgtcggcat tgtgttcttg
      661 tcatttgccc atgatgccgt atttatcagc atcatggtct gggccagtgt caccatggta
      721 cgtctcctgt atagacacta ccagagactg cagtacattt tccctcccca tcaaggcaac
      781 agaggctatg ctgagatcag agcagcccat agcattgtgt tgcttgtggt cacatttgta
      841 agtttttatc ttctacactg tatttgcttt attcttcaca ttgtttttgt agactccaat
      901 ctgtggttga ggcatatagg tgaagtttta acttcaagct tccccactat ttctcccttg
      961 ctgaatcgaa ttcccgcggc cgccatggcg gccgggagca tgcgacgtcg ggcccaattc
     1021 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     1081 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1141 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1201 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1261 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1321 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1381 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1441 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1501 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1561 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1621 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     1681 ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc
     1741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3001 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3661 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3721 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3841 tttcacacag gaaacagcta tgaccatgat tacgccaagc t
//
