LOCUS       sequence-204661-        3887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Vmn1r105
FEATURES             Location/Qualifiers
     source          1..3887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1029..1047)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1030..1047)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1065..1081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1065..1081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1078..1100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1089..1105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1113..1119,1120..1137,1138..1143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1158..1179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1296..1313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1467..2055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1547..1566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2226..3017,3018..3086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2849..2868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3087..3191)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3269..3724)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3406..3427)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3618..3637
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3850..3872
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3864..3881
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3865..3881
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc
       61 ggccgcggga attcgattgg aagtggctct tcagatcctc ttgctttgcc agtttggggt
      121 tgggactgtg gccaatgtct ttctgtttgt ccataatttc tctccagtct tgactggttc
      181 taaacagagg cccagacagg tgattttaag ccacatggct gtggccaatg ccttgactct
      241 attcctcact atatttccaa acaacatgat ggcttttgct cccaaaactc ctccaaatga
      301 actcgaatgt aaattagaat ccttcagtca cctggtggca agaagcacaa acttgtgttc
      361 cacctgtgtc ctgagtgtcc atcagtttgt cactctggtt cctcttaata gagggaaagg
      421 taaacttgta ctcagagcaa gtgtcacaaa catggcaagt tattcttctt acagttattg
      481 gtttttcagt gtcttaagta acatccacat tccaattaag ttcagtggtc cacagatagc
      541 agacaataac actgactcga aaagaaagtt gttctgttcc tcttccggat tcagtgtggg
      601 cattgtcttc ttgcagtttg cgtacgatgc cacattcatg agcatcatgg tctggaccag
      661 tgtctccatg gtacttctcc tccatggaca tcgccagcga atgcagcaca tcctcactcc
      721 caatcagaac cccagaggcc aagctgagtc caaagcaacc catactatcc tgatgctggt
      781 gtttacattt gttagctttt atcttctaaa ttgtatttgt atcatagtac atgccctttt
      841 tatgcattct catttcttcg taaggcttgt cagtgagatt ttaactgcaa tcttccccag
      901 tatctctcct ttactgttga tcttcaggga tcccaatgat ccttgttcaa tcactagtga
      961 attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg gatgcatagc
     1021 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
     1081 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1141 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2821 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag
     3241 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     3301 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3361 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3421 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3481 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3541 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3601 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3661 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3721 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3781 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3841 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattg
//
