LOCUS       sequence-204664-        4641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Walz A, Omura M, Mombaerts P
  TITLE     Development and topography of the lateral olfactory tract in the 
            mouse: imaging by genetically encoded and injected fluorescent 
            markers.
  JOURNAL   J Neurobiol. 2006 Jul . 66(8):835-46.
  PUBMED    16673392
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurobiol. 2006 Jul . 66(8):835-46."
COMMENT     Sequence Label: pISH-Th
FEATURES             Location/Qualifiers
     source          1..4641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1783..1801)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1784..1801)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1819..1835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1819..1835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1832..1854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1843..1859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1867..1873,1874..1891,1892..1897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1912..1933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2050..2067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2221..2809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2301..2320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2980..3771,3772..3840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3603..3622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3841..3945)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4023..4478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4160..4181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4372..4391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4604..4626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4618..4635
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4619..4635
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc
       61 cgcgggatta gcccaagggc ttcagaagag ccgtctcaga gcaggatacc aagcaggccg
      121 aggctgtcac gtccccaagg ttcattggac ggcggcagag tctcatcgag gatgcccgta
      181 aggagcggga ggcagcagca gctgcagcag cggctgcggt agcctccgcg gaacctggga
      241 acccattgga ggctgtggta ttcgaggaga gggatggaaa tgctgttctc aacctgctct
      301 tctccttgag gggtacaaaa ccctcctcac tgtctcgggc tttgaaagtg tttgagacat
      361 ttgaagccaa aatccaccac ttagagaccc ggcctgccca gaggccactg gcaggaagcc
      421 cccacctgga gtactttgtg cgcttcgagg tgcccagtgg cgacctggct gccctcctca
      481 gttctgtgcg tcgggtgtct gacgatgtgc gcagtgccag agaggacaag gttccctggt
      541 tcccaaggaa agtgtcagag ttggataagt gtcaccacct ggtcaccaag tttgaccctg
      601 acctggacct ggaccatccg ggcttctctg accaggcgta tcgccagcgc cggaagctga
      661 ttgcagagat tgccttccaa tacaagcagg gtgagccaat tccccacgtg gaatacacaa
      721 aggaggaaat tgctacctgg aaggaggtat acgccacgct gaagggcctc tatgctaccc
      781 atgcctgccg ggaacacctg gaggctttcc agcttctgga acggtactgt ggctaccgag
      841 aggacagcat tccacagctg gaggatgtgt ctcacttctt gaaggaacgg actggcttcc
      901 agctgcgacc cgtggccggt ctactgtctg cccgtgattt tctggccagt ctggccttcc
      961 gtgtgtttca gtgcacacag tacatccgtc atgcctcctc acctatgcac tcacccgagc
     1021 cagactgctg ccacgagctg ctgggacacg tacccatgtt ggctgaccgc acatttgccc
     1081 agttctccca ggacattgga cttgcatctc tgggggcttc agatgaagaa attgaaaaac
     1141 tctccacggt gtactggttc actgtggagt ttgggctgtg taaacagaat ggggagctga
     1201 aggcttacgg tgcagggctg ctgtcttcct atggagagct cctgcactcc ctgtcagagg
     1261 agcccgaggt ccgggccttt gacccagaca cagcagccgt gcagccctac caagatcaaa
     1321 cctaccagcc ggtgtacttc gtgtcagaga gcttcagtga tgccaaggac aagctcagga
     1381 actatgcctc tcgtatccag cgcccattct ctgtgaagtt tgacccgtac accctggcca
     1441 ttgatgtact ggacagtcct cacaccatcc ggcgctcctt agagggggtc caggatgagc
     1501 tgcacaccct gacccaagca ctgagtgcca ttagctaaat gcatagggta ccacccacag
     1561 gtgccagggg cctttcccaa agttcccagc cccttctcca acctttcctg gcccagaggc
     1621 tttcccatgt gtgtggctgg accctttgat gggctgctct tggtccccct cctccactgc
     1681 ttctcaacca catcttacta ctgcatgcaa tcactagtgc ggccgcctgc aggtcgacca
     1741 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
     1801 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1861 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1921 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2641 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3961 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4021 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4081 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4141 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4201 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4261 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4321 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4381 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4441 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     4501 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     4561 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     4621 aaaacgacgg ccagtgaatt g
//
