LOCUS       sequence-204666-        4066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To prepare riboprobe for in situ hybridization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Trpm5
FEATURES             Location/Qualifiers
     source          1..4066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1208..1226)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1209..1226)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1244..1260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1257..1279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1268..1284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1292..1298,1299..1316,1317..1322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1337..1358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1475..1492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1726..1745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2405..3196,3197..3265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3028..3047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3266..3370)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3448..3903)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3585..3606)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3797..3816
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4029..4051
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4043..4060
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4044..4060
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc
       61 ggccgcggga attcgatttg ttcacactgg tgctggagga aatccgacag ggcttcttca
      121 cagatgagga cacgcacctg gtgaagaaat tcactctgta tgtggaagac aactggaaca
      181 agtgtgacat ggtggccatc ttcctgttca ttgtgggagt cacctgtaga atggtgccct
      241 cggtgtttga ggctggcagg accgttctgg ccattgactt catggtgttc acacttcggc
      301 tcatccacat ctttgctatt cacaagcagt tgggtcctaa gatcatcatt gtagagcgaa
      361 tgatgaagga tgtcttcttt ttcctcttct tcctgagcgt atggcttgtg gcctatggtg
      421 tgaccactca ggccctgctg catccccatg atggccgttt ggagtggatt ttccgccgtg
      481 tgctatacag gccttacctg cagatctttg ggcagatccc tctggatgaa attgatgagg
      541 ctcgtgtgaa ctgttctctt caccctctgc tgctggaagg ctcggcttcc tgccctaatc
      601 tctatgccaa ctggctggtc attctcctgc tggttacctt cctgcttgtc actaatgtgc
      661 tgctcatgaa ccttctgatc gccatgttca gctacacatt ccaggtggtg caaggcaatg
      721 cagacatgtt ctggaagttt caacgctacc acctcatcgt tgaataccat ggaagaccag
      781 ctctggcccc gcccttcatc ctgctcagcc acctgagcct ggtgctcaag caggtcttca
      841 ggaaggaagc ccagcataag cgacaacatc tggagagaga cttgcctgac cccttggacc
      901 agaagatcat tacctgggaa acggttcaaa aggagaactt cctgagtacc atggagaaac
      961 ggaggaggga cagcgagggg gaggtgctga ggaaaacggc acacagagtg gacttgattg
     1021 ccaaatacat cggggggctg agagagcaag aaaagaggat caagtgtctg gaatcacagg
     1081 ccaactactg tatgctcctc ttgtcctcta tgacggatac actggctaat cactagtgaa
     1141 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
     1201 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1321 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1381 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3361 gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga
     3421 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     3481 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3541 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3601 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3661 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3721 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3781 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3841 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3901 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3961 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4021 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattg
//
