LOCUS       sequence-204669-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for
            mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 
            (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-232
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..1180
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1265..1339
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1361..1370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1370..1399
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2138..2194
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2195..2269
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2297..2320
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2761..2770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3085..3105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3090..3419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3270..3405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3429..4028
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3429..3448)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3925..3945
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4321..4554
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4705..4722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4711..4935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4978..4987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5031..5135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5136..5204,5205..5996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5354..5373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6167..6755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6656..6675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6914..7547
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7594..7719
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8212..8445
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8630..8674
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8961..9078
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattgatc cttcgaacta
     1201 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagttcgaag gatccgccgc
     1261 caccatgtcc gtgctgaccc cactcctgct taggggcctt accggctctg ccaggcgcct
     1321 cccggtccca cgggcaaaga tccactcatt gttcgaagcc gccaccatgg aacagaagct
     1381 gatctccgaa gaggacctga tgggcacagc caccatgaca accagtttgg ctacaaagtt
     1441 gagagagggt actaaaaagg ctcacaccat ggcagagaac gtggggttcg tgcggtgttt
     1501 tctgaagggg acagttgaga aaagctctta taggaaactg gtcgcgtcct tgtaccatgt
     1561 ctattccgcg atggagcaag agatggagag attgaaggat cacccaatcg ttgggaagat
     1621 ctattttcct gagctgaatc ggaagagtag tctggagagg gatttgacct attactttgg
     1681 tagcaattgg cgcgaagaga tcccaccctc tccagccact caggcttacg tggcccgaat
     1741 ccatgaggtg gcaaacactg cccccgaact gctggtagca cattcatata ctcgatatct
     1801 tggggatctc tccggaggtc agattcttaa aggcatcgct gagcgagcaa tgaatctgca
     1861 agatggagag ggcactgctt tctatcggtt tgagagcatc tctgacgaga aagcctttaa
     1921 acagctgtat cgacagcggc tcgacgaact gcctgtcgac gaggccaccg cagaccgcat
     1981 cgtcgacgag gctaatgctg ctttcggaat gaacatgaag atctttcagg agctggaagg
     2041 gaatctgatc cgggctatcg gtcaactgct ctttaacact ctgaccaggc ggaagcagag
     2101 ggggtctact gagcttgcta cagccgatgg atctggggcc acaaattttt ccctgctgaa
     2161 acaggctggg gacgtggagg aaaatccagg ccccatgagc gttctcacac ctttgctgct
     2221 ccgggggctc acaggcagtg ctcgacgact gccagtcccg agggcgaaga ttcactcact
     2281 ggctagcgga tcaatggatt acaaggatga cgacgataag tctagcatgg ggactgcaac
     2341 tatgtctctg aggcaacatc aacatcctct catccagaga ctcgcagata gaattgaggc
     2401 aatttggcag gcctttttcc cactggctcc atacgcactg ccggaggact tgggctacgt
     2461 ggaagggaag cttgaaggcg agaggttgac tattgaaaat cactgttatc aggccccgcc
     2521 ttttagaaaa ctgcatttgg agttggcgag ggtcggtgag tctctggata ttttgcactg
     2581 tgtcatgttt cccgaacctc ggtacgacct tcccatgttc ggctgcgatc tggtgggagg
     2641 ccggggccaa atctctgccg caattgttga cctctcacca gtgaccggac agttgccagc
     2701 tgcctacacc tgtgctttga acgcactgcc taagctgaca tttagacagc cccgagagct
     2761 gccaccatgg ggacacattt tttcaccctt ctgcattttt attaggcccc agggcgaggc
     2821 tgaggagcag cagtttttgg atagaatcgg tgaatatctt actctgcact gccagttgtc
     2881 ccaacaggct gtgccaaccg accaccctca agcagtcatt gctggccagc ggcagtattg
     2941 tcagcagcag cagcaaaacg ataagacaag gagggtactg gagaaggcat ttggtgtacc
     3001 ctgggccgag cggtatatga ctactgttct gttcgacgtt ccacccgtgt agtaagaatt
     3061 cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3421 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     3481 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     3541 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     3601 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     3661 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     3721 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     3781 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     3841 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     3901 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     3961 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     4021 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     4081 catcgataaa ataaaagatt ttatttagtc tccagaaaaa gggggcggcc gctcgagacc
     4141 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4201 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4261 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4321 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4381 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4441 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4501 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4561 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4621 agaggccttg acattataat agatttagca ggaattgaac taggagtgga gcacacaggc
     4681 aaagttctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     4741 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4801 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4861 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4921 cggtgggctc tatggcttct gaggcggaaa gaaccagctg ggggcgcgcc cctcgaggcc
     4981 gccatggtca tagctgtttg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcacgaga caataaccct
     5101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6781 ggttctggcc ttttgcacgg accgccactg ccaattacct gtggtttcat ttactctaaa
     6841 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     6901 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     6961 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7021 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7081 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7141 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7201 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7261 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7321 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7561 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7621 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7681 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7741 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7801 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7861 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7921 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7981 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8041 accaccgcac agcaaccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8101 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8161 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8221 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8281 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8341 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8401 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8461 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8521 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8581 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8641 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8701 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8761 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8821 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     8881 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccacaa
     8941 atggcagtat tcatccacaa ttttaaaaga aaggggggga ttggggggta cagtgcaggg
     9001 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9061 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggatcgat
     9121 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     9181 tctaggtctt gaaaggagtg ggaat
//
