LOCUS       sequence-204674-       11751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer 
            EJ, Zhu LJ, Wolfe SA
  TITLE     Orthogonal Cas9-Cas9 chimeras provide a versatile platform for 
            genome editing.
  JOURNAL   Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x.
  PUBMED    30451839
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-19"; volume: "9(1):4856. doi"; pages: " 
            10.1038/s41467-018-07310-x"
COMMENT     Sequence Label: pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
FEATURES             Location/Qualifiers
     source          1..11751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..211
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     193..210
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             261..308
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             327..347
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             354..4454
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4473..4493
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4494..4583
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4596..4622
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             7827..7874
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7893..7919
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     polyA_signal    7961..8095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7985..8004)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8039..8058
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8208..8228)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8208..8226)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8247..8263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8247..8263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8260..8282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8271..8287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8295..8301,8302..8319,8320..8325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8340..8361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8478..8495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8649..9237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8729..8748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9408..10199,10200..10268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10031..10050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10269..10373)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10399..10854)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10536..10557)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10748..10767
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(10925..11751,1..158)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat
       61 ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct
      121 aatgggaggt ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc
      181 ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat
      241 cccatcgatt cgaattcatg aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
      301 agaaaaagct ggacggcagc ggaagcccca agaagaagag gaaggtacca tgggacaaga
      361 agtactccat tgggctcgat atcggtacca acagcgtcgg ctgggccgtc attacggacg
      421 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
      481 agaagaacct cattggagcc ctcctgttcg actccgggga gacggccgaa gccacgcggc
      541 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
      601 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
      661 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
      721 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagctggtag
      781 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcac atgatcaaat
      841 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
      901 tctttatcca actggttcag acttacaatc agcttttcga ggagaacccg atcaacgcat
      961 ccggcgttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa
     1021 acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc
     1081 tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc
     1141 tgcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg
     1201 accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg
     1261 atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc
     1321 gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc
     1381 ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg
     1441 acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg
     1501 acggcaccga ggagctgctg gtaaagctga acagagaaga tctgttgcgc aaacagcgca
     1561 ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca
     1621 ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc
     1681 tcacatttcg gataccctac tatgtaggcc ccctcgctcg gggaaattcc agattcgcgt
     1741 ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata
     1801 agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta
     1861 acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc
     1921 tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc
     1981 agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc
     2041 tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg
     2101 aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca
     2161 aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta
     2221 cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg
     2281 acgacaaagt catgaaacag ctcaagagac gccgatatac aggatggggg cggctgtcaa
     2341 gaaaactgat caatggcatc cgagacaagc agagtggaaa gacaatcctg gattttctta
     2401 agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct
     2461 ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca
     2521 tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg
     2581 tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg
     2641 cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga
     2701 ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca
     2761 cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg
     2821 tggatcagga actggacatc aaccggttgt ccgactacga cgtggatcat atcgtgcccc
     2881 aaagctttct caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata
     2941 gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc
     3001 ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg
     3061 aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga
     3121 cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg
     3181 atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct
     3241 cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg
     3301 cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc
     3361 tggaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa
     3421 agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga
     3481 attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg
     3541 aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc
     3601 gcaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag
     3661 gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa
     3721 aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac
     3781 tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc
     3841 tgggcatcac aatcatggag cgatccagct tcgagaaaaa ccccatcgac tttctcgaag
     3901 cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gctgcccaag tactctctct
     3961 ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta
     4021 acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa
     4081 agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac
     4141 actaccttga tgagatcatc gagcaaataa gcgagttctc caaaagagtg atcctcgccg
     4201 acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg
     4261 agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct
     4321 tcaagtactt cgacaccacc atagacagaa agaagtacac ctctacaaag gaggtcctgg
     4381 acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc
     4441 agctcggtgg agacgctagc ggcaccggcg ggcccaagaa gaagaggaag gtatacccat
     4501 acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcgtatc
     4561 cttatgacgt tccagattac gctggatccg ccgctccggc agctaagaaa aagaaactgg
     4621 atttcgaatc cggaactagt ggtatccacg gagtcccagc agccaagcgg aactacatcc
     4681 tgggcctggc catcggcatc accagcgtgg gctacggcat catcgactac gagacacggg
     4741 acgtgatcga tgccggcgtg cggctgttca aagaggccaa cgtggaaaac aacgagggca
     4801 ggcggagcaa gagaggcgcc agaaggctga agcggcggag gcggcataga atccagagag
     4861 tgaagaagct gctgttcgac tacaacctgc tgaccgacca cagcgagctg agcggcatca
     4921 acccctacga ggccagagtg aagggcctga gccagaagct gagcgaggaa gagttctctg
     4981 ccgccctgct gcacctggcc aagagaagag gcgtgcacaa cgtgaacgag gtggaagagg
     5041 acaccggcaa cgagctgtcc accaaagagc agatcagccg gaacagcaag gccctggaag
     5101 agaaatacgt ggccgaactg cagctggaac ggctgaagaa agacggcgaa gtgcggggca
     5161 gcatcaacag attcaagacc agcgactacg tgaaagaagc caaacagctg ctgaaggtgc
     5221 agaaggccta ccaccagctg gaccagagct tcatcgacac ctacatcgac ctgctggaaa
     5281 cccggcggac ctactatgag ggacctggcg agggcagccc cttcggctgg aaggacatca
     5341 aagaatggta cgagatgctg atgggccact gcacctactt ccccgaggaa ctgcggagcg
     5401 tgaagtacgc ctacaacgcc gacctgtaca acgccctgaa cgacctgaac aatctcgtga
     5461 tcaccaggga cgagaacgag aagctggaat attacgagaa gttccagatc atcgagaacg
     5521 tgttcaagca gaagaagaag cccaccctga agcagatcgc caaagaaatc ctcgtgaacg
     5581 aagaggatat taagggctac agagtgacca gcaccggcaa gcccgagttc accaacctga
     5641 aggtgtacca cgacatcaag gacattaccg cccggaaaga gattattgag aacgccgagc
     5701 tgctggatca gattgccaag atcctgacca tctaccagag cagcgaggac atccaggaag
     5761 aactgaccaa tctgaactcc gagctgaccc aggaagagat cgagcagatc tctaatctga
     5821 agggctatac cggcacccac aacctgagcc tgaaggccat caacctgatc ctggacgagc
     5881 tgtggcacac caacgacaac cagatcgcta tcttcaaccg gctgaagctg gtgcccaaga
     5941 aggtggacct gtcccagcag aaagagatcc ccaccaccct ggtggacgac ttcatcctga
     6001 gccccgtcgt gaagagaagc ttcatccaga gcatcaaagt gatcaacgcc atcatcaaga
     6061 agtacggcct gcccaacgac atcattatcg agctggcccg cgagaagaac tccaaggacg
     6121 cccagaaaat gatcaacgag atgcagaagc ggaaccggca gaccaacgag cggatcgagg
     6181 aaatcatccg gaccaccggc aaagagaacg ccaagtacct gatcgagaag atcaagctgc
     6241 acgacatgca ggaaggcaag tgcctgtaca gcctggaagc catccctctg gaagatctgc
     6301 tgaacaaccc cttcaactat gaggtggacc acatcatccc cagaagcgtg tccttcgaca
     6361 acagcttcaa caacaaggtg ctcgtgaagc aggaagaagc cagcaagaag ggcaaccgga
     6421 ccccattcca gtacctgagc agcagcgaca gcaagatcag ctacgaaacc ttcaagaagc
     6481 acatcctgaa tctggccaag ggcaagggca gaatcagcaa gaccaagaaa gagtatctgc
     6541 tggaagaacg ggacatcaac aggttctccg tgcagaaaga cttcatcaac cggaacctgg
     6601 tggataccag atacgccacc agaggcctga tgaacctgct gcggagctac ttcagagtga
     6661 acaacctgga cgtgaaagtg aagtccatca atggcggctt caccagcttt ctgcggcgga
     6721 agtggaagtt taagaaagag cggaacaagg ggtacaagca ccacgccgag gacgccctga
     6781 tcattgccaa cgccgatttc atcttcaaag agtggaagaa actggacaag gccaaaaaag
     6841 tgatggaaaa ccagatgttc gaggaaaagc aggccgagag catgcccgag atcgaaaccg
     6901 agcaggagta caaagagatc ttcatcaccc cccaccagat caagcacatt aaggacttca
     6961 aggactacaa gtacagccac cgggtggaca agaagcctaa tagagagctg attaacgaca
     7021 ccctgtactc cacccggaag gacgacaagg gcaacaccct gatcgtgaac aatctgaacg
     7081 gcctgtacga caaggacaat gacaagctga aaaagctgat caacaagagc cccgaaaagc
     7141 tgctgatgta ccaccacgac ccccagacct accagaaact gaagctgatt atggaacagt
     7201 acggcgacga gaagaatccc ctgtacaagt actacgagga aaccgggaac tacctgacca
     7261 agtactccaa aaaggacaac ggccccgtga tcaagaagat taagtattac ggcaacaaac
     7321 tgaacgccca tctggacatc accgacgact accccaacag cagaaacaag gtcgtgaagc
     7381 tgtccctgaa gccctacaga ttcgacgtgt acctggacaa tggcgtgtac aagttcgtga
     7441 ccgtgaagaa tctggatgtg atcaaaaaag aaaactacta cgaagtgaat agcaagtgct
     7501 atgaggaagc taagaagctg aagaagatca gcaaccaggc cgagtttatc gcctccttct
     7561 acaacaacga tctgatcaag atcaacggcg agctgtatag agtgatcggc gtgaacaacg
     7621 acctgctgaa ccggatcgaa gtgaacatga tcgacatcac ctaccgcgag tacctggaaa
     7681 acatgaacga caagaggccc cccaggatca ttaagacaat cgcctccaag acccagagca
     7741 ttaagaagta cagcacagac attctgggca acctgtatga agtgaaatct aagaagcacc
     7801 ctcagatcat caaaaagggc cccgggaaaa ggccggcggc cacgaaaaag gccggccagg
     7861 caaaaaagaa aaagctggac ggcagcggaa gcccggcagc taagaaaaag aaactggatc
     7921 tcgagtaata atctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     7981 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     8041 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     8101 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     8161 cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg
     8221 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8281 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
     8341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8461 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8521 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8581 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8641 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8701 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8761 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8821 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8881 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8941 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9001 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9061 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9121 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9181 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9241 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9301 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9361 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9421 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9481 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9541 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9601 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9661 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9721 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9781 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9841 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9901 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9961 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10021 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10081 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10141 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10201 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10261 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10321 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10381 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    10441 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    10501 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    10561 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    10621 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    10681 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    10741 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    10801 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
    10861 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10921 gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca atatggtgga
    10981 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
    11041 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
    11101 cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
    11161 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
    11221 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
    11281 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
    11341 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
    11401 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
    11461 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
    11521 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
    11581 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
    11641 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
    11701 aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat t
//
