LOCUS       sequence-204680-       11132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVCT-Dnd1-EGFP
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             6..639
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    686..811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2061..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2560..2582
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2644..2663
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2722..2839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3943..3952
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3949..3951,3952..3954,3955..4668)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3949..3951,3952..3954,3955..4665)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3994..4015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4255..4274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4602..4623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4717..5305
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4770..4790)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5188..5199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5443..5461,5462..5484,5485..5503,5504..5526,5527..5545,
                     5546..5568,5569..5587,5588..5610,5611..5629,5630..5652,
                     5653..5671,5672..5694,5695..5713)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             5752..5932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5957..5975)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5958..5975)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6265..6284)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6498..6517)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6617..6639
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6677..6695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6763..6867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6868..6936,6937..7728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7086..7105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7899..8487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8388..8407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8641..8658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8728..8748)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8733..9062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8913..9048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10196..10261
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10391..10411
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10836..10970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10873..10892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10927..10946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
       61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
      121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
      181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
      241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
      301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
      361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
      421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gaaggaaatg ggcggggagg
     2461 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     2521 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     2581 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     2641 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatta aatttaatta
     2701 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     2761 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2821 tacaaaaatt caaaatttta tcgatcacga gactagcctc gagccgccac catgcagtcc
     2881 aaacgggagt gcgagcaatg gtgtgagagg gtgaatccag agaacaaggc agccctggag
     2941 gcgtgggtaa gggagactgg catccgcctg gtgcaggtca acgggcaaag gaagtatgga
     3001 gggccacccc caggctgggt gggcagcccg ccaccgtcag ggtccgaggt gtatatcgga
     3061 cgacttccgc aggacgtgta cgagcaccag ctaatcccac tcttccagcg cgtggggcgc
     3121 ctctatgagt tccgcctaat gatgaccttc agtggtttga accgcggctt tgcctatgcg
     3181 cgctacagct cgcggcgcgg tgcccaggcc gccattgcta cgctgcacaa ccaccagctg
     3241 cggccttctt gccagctcct ggtgtgccga agcaccgaga agtgcgagct gacggtggac
     3301 gggctgcctc ttagcttaaa ccgccgagcg ttactgctcg ccctgcaacc gttcggtccc
     3361 tgcctccagg agacactgct gctacccagc cctgggtctg cgccctctca gattgcgctg
     3421 ctcaagttca gtacgcaccg agctgctgct atggccaaaa aggccctggt agaaggtcag
     3481 tcacgcctct gtggagagca ggtggctgtg gagtggctca agccagacct aaagcagcac
     3541 ttccgccagc agcttgcagg tccctcactg cggttcctca ggccagatgt cagccaacta
     3601 acccagacca gggagaagct ggggtcccaa ggggctcggg ctgccctgca actgctgtgt
     3661 caacggatga agctaggcag cccagtgttc ctgaccaagt gtttaggaac agggcctgct
     3721 ggctggcatc gcttttggta ccaggtggtg atccctgggc atccagtgcc tttcagtggc
     3781 ctcatttggg ttgtgttggc ctctgattgg caggatgggc atgaggtggc caaggatgct
     3841 gtgtccgcac agctgctgga ggcactgagt gaacctagga ccagcttgtg gtctcctggg
     3901 gctgaagcag gtactatggt taagcagaag gatccaccgg tcgccaccat ggtgagcaag
     3961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4021 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4141 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     4201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4261 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4321 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4381 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     4441 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     4501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     4561 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4621 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccagctt
     4681 atcgactcgc gaattcgatg ggactagtca tatgataatc aacctctgga ttacaaaatt
     4741 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4801 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4861 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4921 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4981 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5041 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5101 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5161 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5221 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5281 atctcccttt gggccgcctc cccgcatcgg tacgtatggc caggtacctt taagaccaat
     5341 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5401 gctaattcac tcccaacgaa gacaagatct cgagtttacc actccctatc agtgatagag
     5461 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     5521 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     5581 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     5641 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     5701 tcagtgatag agaaaagtga aagtcggatc gatctgcttt ttgcttgtac tgggtctctc
     5761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     5941 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     6001 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     6061 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     6121 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     6181 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     6241 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     6301 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     6361 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     6421 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6481 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6541 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6601 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6661 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7081 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8701 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8821 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9001 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9061 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     9121 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     9181 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     9241 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     9301 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     9361 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     9421 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     9481 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     9541 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     9601 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     9661 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     9721 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     9781 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     9841 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     9901 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
     9961 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    10021 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    10081 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    10141 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    10201 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    10261 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    10321 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    10381 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    10441 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    10501 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    10561 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    10621 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    10681 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    10741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    10801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    10861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10981 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11041 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11101 attgtatttg ttaaatatgt actacaaact ta
//
