LOCUS       sequence-204697-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for
            mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and 
            Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-234
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..1180
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1280..1354
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1376..1385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1385..1414
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2144..2200
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2201..2275
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2303..2326
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3097..3117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3102..3431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3282..3417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3441..4040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3441..3460)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3937..3957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4333..4566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4717..4734)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4723..4947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4990..4999
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5043..5147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5148..5216,5217..6008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5366..5385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6179..6767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6668..6687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6926..7559
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7606..7731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8224..8457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8642..8686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8973..9090
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattgatc cttcgaacta
     1201 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagttcgaag gatccgctag
     1261 cacattcgaa gccgccacca tgtccgtctt gacaccattg ttgttgagag ggttgacagg
     1321 ttcagccagg agattgccag tgcccagagc caaaatacat tcattattcg aagccgccac
     1381 catggaacag aagctgatct ccgaagagga cctgatggtc gccaacctgg ccaccatgct
     1441 gagggagggc accaagacaa gtcacaccat ggccgagaat gtcggattcg tgaaatgctt
     1501 tctcaagggt gttgtggaaa agaagtccta caggaagctg gtggctgacc tgtattacgt
     1561 gtactcagcc atggaagagg agatggagag actgaaagat catcctgtcg tgtcccagat
     1621 ctattttcca gaactcaaca ggaagcagtc actggagaca gacctgagat attacttcgg
     1681 acccaattgg caggccgagg ccaaaattac ccccgcagga caggcctatg tggacaggat
     1741 tcatgaggtg gctcagaccg cccctgaact cctggtctca cactcctata cccgctatct
     1801 cggcgacctg agcggaggcc agatcctgaa gaagatcgca cagaacgcaa tgaatctgga
     1861 cggggaggga accgctttct acgagtttga gaatatcagt gacgagaaag ccttcaaaga
     1921 taagtaccgg gcagcaatga atagcctgga tgtgccagag gaaactgctg aacagatcgt
     1981 gcaggaagcc aatgatgcct tcggcatgaa catgaatatg tttaaggagc tggaaggtaa
     2041 tctggtcaag gccatcggcg tcatgctgtt taataccctg acacgccgga gaaccaaggg
     2101 cagcacagac gcagacctgt cacctgcttc taatggaagc ggagctacta acttcagcct
     2161 gctgaagcag gctggagacg tggaggagaa ccctggacct atgagtgttc tgaccccatt
     2221 gctgttgaga ggactgactg gttccgccag gaggttgccc gtgccaagag ccaaaataca
     2281 tagcttagct agcggatcca tggattacaa ggatgacgac gataagtcta gcatgactgc
     2341 tcctgccaca aaacctaagt tttaccctct gattgagcag ctggcaggag tgatcctgga
     2401 gacctggcac cagcatctgg agctggaacc ctttaccctg cctgaaggac tgggctatat
     2461 tgaaggcaag ctggaaggcg agagactgac tattgagaac acctgttacc agagccagca
     2521 cttcaggaag atgcacctgg agatggccaa agtgggagaa aacctggaca tcctgcactg
     2581 cgtcatgttt cccagggccg aatatgctct gcccatgttc ggctgtgacc tggtcggcaa
     2641 tccaagaggc gtgagtgccg ctattgcaga cctgtctcct acctccgcag acaagactct
     2701 gtccgtggac tatcagacaa ggctgagaca gaaccagggg aatctgaact tcagtcagcc
     2761 aagagacctg cccgaatggg gagcaatctt cagcgagttt tgcctgttta tccggccaag
     2821 taatcccaac gaagagcagc agtttctgga gcgcgtgaga gagttcctga caatccattg
     2881 tcagctggcc ctgggcctgg aaaagctgag cccagcagaa caggctatct atctggctgg
     2941 ccagaggcac tattgccagg agcagcagag gaatgacaaa accaggagag tgctggagaa
     3001 agcctttaac cccgattggg cagagcgata catgagccag gtgctgtttg acatccccgc
     3061 ctaataagaa ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3421 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3481 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3541 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3601 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     3661 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     3721 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     3781 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     3841 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     3901 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     3961 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4021 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     4081 cacgacccca tgcatcgata aaataaaaga ttttatttag tctccagaaa aagggggcgg
     4141 ccgctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     4201 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     4261 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4321 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4561 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4621 tatcagagag tgagaggcct tgacattata atagatttag caggaattga actaggagtg
     4681 gagcacacag gcaaagttct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4741 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4801 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4861 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4921 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgggggcgcg
     4981 cccctcgagg ccgccatggt catagctgtt tgacgtcagg tggcactttt cggggaaatg
     5041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcacga
     5101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5281 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5701 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6781 cggccttttt acggttctgg ccttttgcac ggaccgccac tgccaattac ctgtggtttc
     6841 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     6901 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     6961 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     7021 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     7081 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     7141 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     7201 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     7261 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     7321 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     7381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7561 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7621 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7681 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7741 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7801 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7861 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7921 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7981 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8041 aacaaaagta agaccaccgc acagcaaccg gccgctgatc ttcagacctg gaggaggaga
     8101 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8161 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8221 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8281 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8341 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8401 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8461 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8521 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8581 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8641 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8701 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8761 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8821 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     8881 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     8941 gtatcgccac aaatggcagt attcatccac aattttaaaa gaaagggggg gattgggggg
     9001 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9061 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     9121 ctttggatcg ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca
     9181 gctaatggac cttctaggtc ttgaaaggag tgggaat
//
