LOCUS       sequence-204699-       10856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HACK Donor plasmids for converting GAL4 lines to GAL80 lines using 
            HACK method..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10856)
  TITLE     Potter Lab plasmids
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHACK-GAL4>GAL80
FEATURES             Location/Qualifiers
     source          1..10856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..22
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    229..262
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    276..309
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    478..511
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1720..1773
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1780..3087
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     intron          3175..3240
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3370..3390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3662..3796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3803..3836)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3900..4021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4141..4851)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4688..4706
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(5029..5062)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6097..6441
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    6451..6484
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        6500..7296
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        7316..7391
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7392..7812
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7833..7908
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8341..8374)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8844..8861)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8844..8860)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8959..8976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9130..9718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9210..9229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9840..10631,10632..10700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10463..10482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10701..10792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10834..10853
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atatggctca taacacccct tgtttgcctg gcggcagtag cgcggtggtc ccacctgacc
       61 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg actccccatg
      121 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      181 tttcgcccgg gctaattatg gggtgtcgcc cttattcgac tctatagtga agttcctatt
      241 ctctagaaag tataggaact tctgaagtgg ggcgggtgcc agggcgtgcc cttgggctcc
      301 ccgggcgcgt acaagccgaa ggcgcgcatg atgaaataac ataaggtggt cccgtcgaaa
      361 gccgaagctt accgaagtat acacttaaat tcagtgcacg tttgcttgtt gagaggaaag
      421 gttgtgtgcg gacgaatttt tttttgaaaa cattaaccct tacgtaacgc gagatctgaa
      481 gttcctattc tctagaaagt ataggaactt ctagggataa cagggtaata gatctggatc
      541 ctaatgatga catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg
      601 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg
      661 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag
      721 ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta
      781 caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc
      841 gtcacagata gattggcttc agtggagact gatatgcctc taacattgag acagcataga
      901 ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta
      961 tcgattgact cggcagctca tcatgataac tccacaattc cgttggattt tatgcccagg
     1021 gatgctcttc atggatttga ttggtctgaa gaggatgaca tgtcggatgg cttgcccttc
     1081 ctgaaaacgg accccaacaa taatgggttc tttggcgacg gttctctctt atgtattctt
     1141 cgatctattg gctttaaacc ggaaaattac acgaactcta acgttaacag gctcccgacc
     1201 atgattacgg atagatacac gttggcttct agatccacaa catcccgttt acttcaaagt
     1261 tatctcaata attttcaccc ctactgccct atcgtgcact caccgacgct aatgatgttg
     1321 tataataacc agattgaaat cgcgtcgaag gatcaatggc aaatcctttt taactgcata
     1381 ttagccattg gagcctggtg tatagagggg gaatctactg atatagatgt tttttactat
     1441 caaaatgcta aatctcattt gacgagcaag gtcttcgagt caggttccat aattttggtg
     1501 acagccctac atcttctgtc gcgatataca cagtggaggc agaaaacaaa tactagctat
     1561 aattttcaca gcttttccat aagaatggcc atatcattgg gcttgaatag ggacctcccc
     1621 tcgtccttca gtgatagcag cattctggaa caaagacgcc gaatttggtg gtctgtctac
     1681 tcttgggaga tccaattgtc cctgcttgga tcctcgcgtg agggccgcgg cagcctgctg
     1741 acctgcggcg atgtggagga gaaccccggg ccccctagga tggactacaa caagagatct
     1801 tcggtctcaa ccgtgcctaa tgcagctccc ataagagtcg gattcgtcgg tctcaacgca
     1861 gccaaaggat gggcaatcaa gacacattac cccgccatac tgcaactatc gtcacaattt
     1921 caaatcactg ccttatacag tccaaaaatt gagacttcta ttgccaccat tcagcgtcta
     1981 aaattgagta atgccactgc ttttcccact ttagagtcat ttgcatcatc ttccactata
     2041 gatatgatag tgatagctat ccaagtggcc agccattatg aagttgttat gcctctcttg
     2101 gaattctcca aaaataatcc gaacctcaag tatcttttcg tagaatgggc ccttgcatgt
     2161 tcactagatc aagccgaatc catttataag gctgctgctg aacgtggggt tcaaaccatc
     2221 atctctttac aaggtcgtaa atcaccatat attttgagag caaaagaatt aatatctcaa
     2281 ggctatatcg gcgacattaa ttcgatcgag attgctggaa atggcggttg gtacggctac
     2341 gaaaggcctg ttaaatcacc aaaatacatc tatgaaatcg ggaacggtgt agatctggta
     2401 accacaacat ttggtcacac aatcgatatt ttacaataca tgacaagttc gtacttttcc
     2461 aggataaatg caatggtttt caataatatt ccagagcaag agctgataga tgagcgtggt
     2521 aaccgattgg gccagcgagt cccaaagaca gtaccggatc atcttttatt ccaaggcaca
     2581 ttgttaaatg gcaatgttcc agtgtcatgc agtttcaaag gtggcaaacc taccaaaaaa
     2641 tttaccaaaa atttggtcat tgacattcac ggtaccaagg gagatttgaa acttgaaggc
     2701 gatgccggct tcgcagaaat ttcaaatctg gtcctttact acagtggaac tagagcaaac
     2761 gacttcccgc tagccaatgg acaacaagct cctttagacc cggggtatga tgcaggtaaa
     2821 gaaatcatgg aagtatatca tttacgaaat tataatgcca ttgtgggtaa tattcatcga
     2881 ctgtatcaat ctatctctga cttccacttc aatacaaaga aaattcctga attaccctca
     2941 caatttgtaa tgcaaggttt cgatttcgaa ggctttccca ccttgatgga tgctctgata
     3001 ttacacaggt taatcgagag cgtttataaa agtaacatga tgggctccac attaaacgtt
     3061 agcaatatct cgcattatag tttataagac gtctagagga tctttgtgaa ggaaccttac
     3121 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     3181 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     3241 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     3301 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     3361 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     3421 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     3481 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat
     3541 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3601 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3661 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3781 ttatcatgtc tggatcgcat gcataacttc gtatagcata cattatacga agttatggta
     3841 ccactcgacg attaaccgtt agtttagcaa gtataattga attttttata agatccagct
     3901 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3961 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4021 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4081 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcaga gtcgcggcgc
     4141 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
     4201 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
     4261 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
     4321 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     4381 ggggtacatc cgctcggagg aggcctccca gcccatggtt ttcttctgca ttacggggcc
     4441 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     4501 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     4561 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     4621 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     4681 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     4741 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     4801 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tgggccgatt
     4861 gtttagcttg ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg acgtgttcac
     4921 tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc gcctctattt atactccggc
     4981 gctaattgaa ttagctctaa ttgaattagt ctctaattga atgacgtcat aacttcgtat
     5041 agcatacatt atacgaagtt atagatcact agtcatggca tcattgaaac agcaaggctc
     5101 ttacaagttt tcacaaaaat ctatgaacta gacaaaacag taactgcaga aaaaagtcct
     5161 atatgtgcaa aaaaatgctt gatgatttgt aatgagattg aggaggtttc gagacaggca
     5221 ccaaagtttt tacaaatgga tatttccacc accgctctaa ccaatttgtt gaaggaacac
     5281 ccttggctat cctttacaag attcgaactg aagtggaaac agttgtctct tatcatttat
     5341 gtattaagag attttttcac taattttacc cagaaaaagt cacaactaga acaggatcaa
     5401 aatgatcatc aaagttatga agttaaacga tgctccatca tgttaagcga tgcagcacaa
     5461 agaactgtta tgtctgtaag tagctatatg gacaatcata atgtcacccc atattttgcc
     5521 tggaattgtt cttattactt gttcaatgca gtcctagtac ccataaagac tctactctca
     5581 aactcaaaat cgaatgctga gaataacgag accgcacaat tattacaaca aattaacact
     5641 gttctgatgc tattaaaaaa actggccact tttaaaatcc agacttgtga aaaatacatt
     5701 caagtactgg aagaggtatg tgcgccgttt ctgttatcac agtgtgcaat cccattaccg
     5761 catatcagtt ataacaatag taatggtagc gccattaaaa atattgtcgg ttctgcaact
     5821 atcgcccaat accctactct tccggaggaa aatgtcaaca atatcagtgt taaatatgtt
     5881 tctcctggct cagtagggcc ttcacctgtg ccattgaaat caggagcaag tttcagtgat
     5941 ctagtcaagc tgttatctaa ccgtccaccc tctcgtaact ctccagtgac aataccaaga
     6001 agcacacctt cgcatcgctc agtcacgcct tttctagggc aacagcaaca gctgcaatca
     6061 ttagtgccac tgaccccgtc tgctttgttt ggtggcgcca attttaatca aagtgggaat
     6121 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
     6181 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
     6241 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
     6301 ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
     6361 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
     6421 ccaaacccaa aaaaagagta atagactagt gaagttccta ttctctagaa agtataggaa
     6481 cttctagtga tctgaattca ttttcaacgt cctcgatagt atagtggtta gtatccccgc
     6541 ctgtcacgcg ggagaccggg gttcaattcc ccgtcgggga gaatctgtga ttcttttttt
     6601 ttttctttta ctttgttata taaacaattt ttgttttaat tgaatctaat ttgccattgc
     6661 ttttaggaat ctcaggcatc cagcaagcgt ttgtccgccg aatcgcccat cagtgaagaa
     6721 gatcctgtgg cggctacgaa aatctccccg gccatgtcgg cctccacctc cagcgaaaaa
     6781 cccatcagcg agctggccac ctctgtgctg acccaccgct ttccagactc cacctcctca
     6841 cccggcgaac atggccttgg acgaatgcag ttgtcgatcc gctacagcgc ccagcgtcaa
     6901 aaactagacg tgaccataca caaaatccag aagataccac ttcgcgatcc cagcaatatc
     6961 cccgatccgt atgttaagct gtatctgttg cctggacgca ccaaggagtc gaaacgcaag
     7021 acgagcgtga tcaaggacaa ctgcaacccc gtctacgatg catcctttga gtacctgatt
     7081 tccattgccg aactcaggca gacggaactg gaggtgacgg tgtgcaccca aaagggattc
     7141 ctatccggcg gtagtcccat cattggcatg gtaggtaccc gaaagcaacc ccttagttac
     7201 agacacagcg cgtacgtcct tcgcatcctt atgattccca agtacatatt ctgcaagagt
     7261 acagtatata taggaaagat atccgggtga acttcgatgt gcagcgtacc acaacgtttt
     7321 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     7381 cgagtcggtg cttttttgct cacctgtgat tgctcctact caaatacaaa aacatcaaat
     7441 tttctgtcaa taaagcatat ttatttatat ttattttaca ggaaagaatt ccttttaaag
     7501 tgtattttaa cctataatga aaaacgatta aaaaaaatac ataaaataat tcgaaaattt
     7561 ttgaatagcc caggttgata aaaattcatt tcatacgttt tataacttat gcccctaagt
     7621 attttttgac catagtgttt caattctaca ttaattttac agagtagaat gaaacgccac
     7681 ctactcagcc aagaggcgaa aaggttagct cgccaagcag agagggcgcc agtgctcact
     7741 actttttata attctcaact tctttttcca gactcagttc gtatatatag acctattttc
     7801 aatttaacgt cgtgtattct gagaaagctg gagttttaga gctagaaata gcaagttaaa
     7861 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgcctac
     7921 ctggagcctg agagttgttc aataaaataa aaatgtttcg tttttttgct ttcgccagta
     7981 tttattattt ttcatcaata tgtattcaat ttggtatgta tttagtaatt gtaatatata
     8041 gacaatggtt ttccgttgac gtacatacat ctgacgtgtg tttatttaga cataatagtt
     8101 atgttttcac atctttttaa tgttcgctta atgcgtatgc ttaattaatt ctgacgtaag
     8161 ctagttacaa accccacgga catgctaagg gttaatcaac aatcatatcg ctgtctcact
     8221 cagactcaat acgacactca gaatactatt cctttcactc gcacttattg caagcatacg
     8281 ttaagtggat gtctcttgcc gacgggacca ccttatgtta tttcatcatg aaaatgaagt
     8341 gaagttccta tactttctag agaataggaa cttctatagt gagtcgaata agggcgacac
     8401 aaaatttatt ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt
     8461 gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc
     8521 gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat
     8581 aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta
     8641 tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa
     8701 agtgacaggc gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa
     8761 aaacccgccg aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac
     8821 cgcccagggg gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag
     8881 aaacgcaaaa aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct
     8941 actctcgcct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9541 gtggtgggct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca
     9781 cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct
     9841 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9901 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9961 agcgctgcga tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac
    10021 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10081 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10141 gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10201 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10261 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10321 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10381 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10441 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10501 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10561 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10621 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10681 cggaaatgtt gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt
    10741 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc
    10801 agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat acctga
//
