LOCUS       sequence-20470-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..171)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(179..195)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(179..195)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(201..464)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(284..307)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 agccggaagc taaagtgtaa agcctggggg tgcctaatgc ggccgccata gtgactggat
       61 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      121 gatatttata tcattttacg tttctcgttc agcttttttg tacaaacttg tgatggggga
      181 tccactagtt ctagaatccg tcgaaactaa gttctggtgt tttaaaacta aaaaaaagac
      241 taactataaa agtagaattt aagaagttta agaaatagat ttacagaatt acaatcaata
      301 cctaccgtct ttatatactt attagtcaag taggggaata atttcaggga actggtttca
      361 accttttttt tcagcttttt ccaaatcaga gagagcagaa ggtaatagaa ggtgtaagaa
      421 aatgagatag atacatgcgt gggtcaattg ccttgtgtca tcatttactc caggcaggtt
      481 gcatcactcc attgaggttg tgcccgtttt ttgcctgttt gtgcccctgt tctctgtagt
      541 tgcgctaaga gaatggacct atgaactgat ggttggtgaa gaaaacaata ttttggtgct
      601 gggattcttt ttttttctgg atgccagctt aaaaagcggg ctccattata tttagtggat
      661 gccaggaata aactgttcac ccagacacc
//
