LOCUS       sequence-204704-       11559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - 
            EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A 
            - MTS - tFNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 11559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-248
FEATURES             Location/Qualifiers
     source          1..11559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1264..1338
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2731..2805
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3553..3609
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3610..3684
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3706..3715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3715..3756
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4066..4122
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4123..4197
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             4228..4245
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5438..5458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5443..5772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5623..5758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5782..6381
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5782..5801)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6278..6298
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6674..6907
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7058..7075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7064..7288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7331..7340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7384..7488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7489..7557,7558..8349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7707..7726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8520..9108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9009..9028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             9267..9900
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9947..10072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10565..10798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10983..11027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11314..11431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgccgcc
     1261 accatgtccg tgctgacccc actcctgctt aggggcctta ccggctctgc caggcgcctc
     1321 ccggtcccac gggcaaagat ccactcattg atgtctctga gacagcacca gcaccccctg
     1381 atccagagac tggccgacag aatcgaggcc atctggcagg cctttttccc cctggcccct
     1441 tacgccctgc ctgaggatct gggctacgtg gaaggcaagc tggaaggcga gcggctgacc
     1501 atcgagaacc actgctacca ggcccctcca ttccggaagc tgcacctgga actggccaga
     1561 gtgggagagt ccctggacat cctgcactgc gtgatgttcc ccgagcccag atacgacctg
     1621 cccatgttcg gctgcgacct cgtgggaggc agaggccaga tctctgccgc catcgtggat
     1681 ctgtctcccg tgaccggaca gctgcctgcc gcttacacct gtgccctgaa tgccctgccc
     1741 aagctgacct tccggcagcc cagagaactg cctccttggg gccacatctt cagccctttc
     1801 tgcatcttca tccggcccca gggcgaggcc gaggaacagc agttcctgga tcggatcggc
     1861 gagtacctga ccctgcactg tcagctgtcc cagcaggccg tgcctaccga tcatccacag
     1921 gctgtgatcg ccggccagag acagtactgc caacagcagc agcagaacga caagaccaga
     1981 cgggtgctgg aaaaggcctt cggcgtgcca tgggccgagc ggtatatgac caccgtgctg
     2041 ttcgacgtgc cccccgtgtg aaagcttgat atccagcaca gtggcggccg caagcttaaa
     2101 acagctctgg ggttgtaccc accccagagg cccacgtggc ggctagtact ccggtattgc
     2161 ggtacccttg tacgcctgtt ttatactccc ttcccgtaac ttagacgcac aaaaccaagt
     2221 tcaatagaag ggggtacaaa ccagtaccac cacgaacaag cacttctgtt tccccggtga
     2281 tgtcgtatag actgcttgcg tggttgaaag cgacggatcc gttatccgct tatgtacttc
     2341 gagaagccca gtaccacctc ggaatcttcg atgcgttgcg ctcagcactc aaccccagag
     2401 tgtagcttag gctgatgagt ctggacatcc ctcaccggtg acggtggtcc aggctgcgtt
     2461 ggcggcctac ctatggctaa cgccatggga cgctagttgt gaacaaggtg tgaagagcct
     2521 attgagctac ataagaatcc tccggcccct gaatgcggct aatcccaacc tcggagcagg
     2581 tggtcacaaa ccagtgattg gcctgtcgta acgcgcaagt ccgtggcgga accgactact
     2641 ttgggtgtcc gtgtttcctt ttattttatt gtggctgctt atggtgacaa tcacagattg
     2701 ttatcataaa gcgaattgga attcgccacc atgtccgtcc tgacgccgct gctgctgcgg
     2761 ggcttgacag gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggt
     2821 accgccacca tgacaacatc tctggccacc aagctgagag agggcaccaa gaaagcccac
     2881 accatggccg agaacgtggg cttcgtgcgg tgcttcctga agggaaccgt ggaaaagtcc
     2941 tcctaccgga agctggtggc ctccctgtac cacgtgtact ccgccatgga acaggaaatg
     3001 gaacggctga aggaccaccc catcgtgggc aagatctact tccccgagct gaaccggaag
     3061 tcctccctgg aacgggacct gacctactac ttcggctcca actggcggga agagatcccc
     3121 ccatctcctg ccacccaggc ctacgtggcc agaatccacg aggtggccaa taccgcccct
     3181 gagctgctgg tggctcactc ctacaccaga tacctgggcg acctgtctgg cggccagatt
     3241 ctgaagggca ttgccgagcg ggccatgaac ctgcaggatg gcgagggcac cgccttctac
     3301 agattcgagt ccatctccga cgagaaggcc tttaagcagc tgtaccggca gcggctggac
     3361 gagctgcctg tggatgaggc caccgccgac agaatcgtgg acgaggccaa tgccgccttc
     3421 ggcatgaaca tgaagatctt tcaggaactg gaaggcaacc tgatccgggc catcggccag
     3481 ctgctgttca acaccctgac ccggcggaag cagagaggct ctaccgagct ggccaccgct
     3541 gacggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     3601 cctggaccta tgagcgtgct gacgcccttg ctgcttagag gactgaccgg aagcgcacgc
     3661 cgcctgcccg tgcctagggc gaagatacat agcctgttcg aagccgccac catgggtaag
     3721 cccattccca acccgctgtt gggcctcgat agcacagtta acatggctac ctacaaagtg
     3781 acactggttc ggcctgatgg tagcgagaca acaattgacg tgcctgagga cgaatacata
     3841 ctcgacgttg cggaagagca agggctggat ctgccattta gttgccgagc gggcgcttgc
     3901 agcacttgtg ccgggaagtt gctcgaaggg gaagtggatc agtctgatca gtcctttctg
     3961 gatgacgatc agatcgaaaa agggttcgtg ctcacctgtg tcgcttatcc aaggagcgat
     4021 tgtaagattc tcactaatca ggaggaggag ctttacggct caggcgcgac aaatttttct
     4081 ctcctcaaac aggctgggga cgtggaggag aaccctggcc ctatgtccgt tcttacccca
     4141 ctgttgttgc ggggactcac tggatcggct agacgactcc ccgtcccaag ggcaaagatc
     4201 cattctctgg aattcgccgc caccatgcac caccaccatc accacgttaa catgtacaat
     4261 gcaaccaact ccaggagcag aatgtttaga tatgaagtag taggactgag gcagactgcc
     4321 gaaaccgaaa agaccaacta tgccatacga aatagcggaa gccagttttt taacgtaccc
     4381 tatgatagga tgaatcagtt catgcagcag ataacacgct ggggcggaaa gatcgtatcc
     4441 attcagccct tgaacggcac agtggctccc ctggctgcca ccacggaacc cgctgcaaat
     4501 aatggcgctg cgcccgtcaa agagaaaaag gttgatatac cagttaacat ctatcgcccg
     4561 aacaacccct gtattggaaa agtaatatcc aacgaggaac tggtgaggga gggcggtgag
     4621 ggaaccgtga aacacattat ttttgatatc tctggaaccg agctcagata tttggagggc
     4681 cagtctatcg ggatcattcc ggctgggacc gacgcaaatg gtaaaccaca taaactccgg
     4741 ctgtattcta tcgcctcaac gaggcacggg gactttcagg atgacaagac cgtctcactg
     4801 tgcgttagac gcctcgaata taaagataaa gagactggcg agaccattta tggcgtgtgc
     4861 tcctcctacc tgaatcagct gcagcctgga gacgaagtga agattactgg tcccgttggg
     4921 aaggaaatgc tgttgagtga cgaccctgaa gccaccatca taatgcttgc taccggcacc
     4981 ggaattgccc cctttcgcgc tttcttgtgg cggatgttca aagaaaacaa cccagattat
     5041 cagttcaaag gacttgcctg gctgtttttt ggcgtcgcct acaccgctaa catcctgtat
     5101 aaagatgagc tggaggccat ccaggcccag tatcctgatc actttcggct tacatacgca
     5161 atttcacgag agcagaagac tccggacggt gggaaaatgt acatccaggg gcgaatcgcc
     5221 gaacatgctg acgagatttg gcagttgctt cagaaaaaaa acacacacgt ctatatgtgc
     5281 ggcctgaggg gcatggaacc tgggatagat gaagcaatga cagcagccgc cgccaagaat
     5341 ggcgcagatt ggcaggaatt tcttaaagga acattgaaga aagagggacg gtggcacgtg
     5401 gaaacatatt aataaccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt
     5461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5521 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5581 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5701 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5761 ggcttttgca aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5821 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5881 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5941 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6001 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6061 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6121 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6181 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6241 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6301 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6361 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     6421 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggcg
     6481 gccgctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6541 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6601 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6661 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6901 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6961 atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg aactaggagt
     7021 ggagcacaca ggcaaagttc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     7081 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7141 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7201 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7261 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcgc
     7321 gcccctcgag gccgccatgg tcatagctgt ttgacgtcag gtggcacttt tcggggaaat
     7381 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcacg
     7441 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7501 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7561 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7621 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7681 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7741 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7801 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7861 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7921 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7981 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8041 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8101 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8161 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8221 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8281 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8341 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8401 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8461 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8641 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9121 gcggcctttt tacggttctg gccttttgca cggaccgcca ctgccaatta cctgtggttt
     9181 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9241 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     9301 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     9361 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9421 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9481 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9541 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     9601 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9661 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9901 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9961 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10021 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10081 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    10141 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    10201 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    10261 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    10321 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    10381 aaacaaaagt aagaccaccg cacagcaacc ggccgctgat cttcagacct ggaggaggag
    10441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    10501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    10561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    10621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    10681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    10741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    10801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    10861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    10921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    10981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    11041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    11101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
    11161 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
    11221 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
    11281 ggtatcgcca caaatggcag tattcatcca caattttaaa agaaaggggg ggattggggg
    11341 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    11401 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    11461 actttggatc gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
    11521 agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
