LOCUS       sequence-204712-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Quarato G, Guy CS, Grace CR, Llambi F, Nourse A, Rodriguez DA, 
            Wakefield R, Frase S, Moldoveanu T, Green DR
  TITLE     Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding 
            Sites Executes Necroptosis.
  JOURNAL   Mol Cell. 2016 Feb 18;61(4):589-601. doi: 
            10.1016/j.molcel.2016.01.011. Epub 2016 Feb 4.
  PUBMED    26853145
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.01.011"; journalName: "Mol Cell"; date: 
            "2016-02-18- 18"; volume: "61"; issue: "4"; pages: "589-601"
COMMENT     Sequence Label: hMLKL-Venus-Plasma
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             719..894
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    957..1156
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1357..1773
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1703..1725
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1741..1757
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1813..2162
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1821..1839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1857..1875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1893..1911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1929..1947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1965..1983
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2001..2019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2037..2055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2101..2125
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3600..3602,3603..3605,3606..4316)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3645..3666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3906..3925)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4253..4274
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4411..4910
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4446..4469)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4843..4862
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4931..5530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4931..4950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5427..5447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5711..6136
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6239..6261)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6397..6417)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6402..6731
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6582..6717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6753..6772
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6853..6870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7024..7612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7104..7123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7783..8574,8575..8643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8406..8425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8644..8748)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttg
       61 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      121 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      661 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca
      721 ataaaagagc ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg
      781 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
      841 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
      901 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      961 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     1021 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1081 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1141 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1201 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1261 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1321 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1381 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1441 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1501 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1561 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1621 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1681 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1741 ctttatccag ccctcactcc ttctctaggc gccggaattg aagatctcga tgaggccctt
     1801 tcgtcttcac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1861 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1921 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     1981 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     2041 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggcgcct ataaaagcag
     2101 agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac
     2161 ttggatccat cgataccatg gaaaatttga agcatattat cacccttggc caggtcatcc
     2221 acaaacggtg tgaagagatg aaatactgca agaaacagtg ccggcgcctg ggccaccgcg
     2281 tcctcggcct gatcaagcct ctggagatgc tccaggacca aggaaagagg agcgtgccct
     2341 ctgagaagtt aaccacagcc atgaaccgct tcaaggctgc cctggaggag gctaatgggg
     2401 agatagaaaa gttcagcaat agatccaata tctgcaggtt tctaacagca agccaggaca
     2461 aaatactctt caaggacgtg aacaggaagc tgagtgatgt ctggaaggag ctctcgctgt
     2521 tacttcaggt tgagcaacgc atgcctgttt cacccataag ccaaggagcg tcctgggcac
     2581 aggaagatca gcaggatgca gacgaagaca ggcgagcttt ccagatgcta agaagagata
     2641 atgaaaaaat agaagcttca ctgagacgat tagaaatcaa catgaaagaa atcaaggaaa
     2701 ctttgaggca gtatttacca ccaaaatgca tgcaggagat cccgcaagag caaatcaagg
     2761 agatcaagaa ggagcagctt tcaggatccc cgtggattct gctaagggaa aatgaagtca
     2821 gcacacttta taaaggagaa taccacagag ctccagtggc cataaaagta ttcaaaaaac
     2881 tccaggctgg cagcattgca atagtgaggc agactttcaa taaggagatc aaaaccatga
     2941 agaaattcga atctcccaac atcctgcgta tatttgggat ttgcattgat gaaacagtga
     3001 ctccgcctca attctccatt gtcatggagt actgtgaact cgggaccctg agggagctgt
     3061 tggataggga aaaagacctc acacttggca agcgcatggt cctagtcctg ggggcagcac
     3121 gaggcctata ccggctacac cattcagaag cacctgaact ccacggaaaa atcagaagct
     3181 caaacttcct ggtaactcaa ggctaccaag tgaagcttgc aggatttgag ttgaggaaaa
     3241 cacagacttc catgagtttg ggaactacga gagaaaagac agacagagtc aaatctacag
     3301 catatctctc acctcaggaa ctggaagatg tattttatca atatgatgta aagtctgaaa
     3361 tatacagctt tggaatcgtc ctctgggaaa tcgccactgg agatatcccg tttcaaggct
     3421 gtaattctga gaagatccgc aagctggtgg ctgtgaagcg gcagcaggag ccactgggtg
     3481 aagactgccc ttcagagctg cgggagatca ttgatgagtg ccgggcccat gatccctctg
     3541 tgcggccctc tgtggatgaa atcttaaaga aactctccac cttttctaag gcggccgcaa
     3601 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3721 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3781 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     3841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3901 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4021 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     4081 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     4141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4201 acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4261 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtcta
     4321 gacagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc tgcatgagct
     4381 gcaagtgtgt gctctcctga gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     4441 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     4501 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     4561 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     4621 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     4681 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     4741 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     4801 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     4861 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     4921 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4981 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5041 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5101 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5161 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5221 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5281 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5341 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5401 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5461 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5521 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     5581 tgcatcggcg tctcgagata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     5641 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     5701 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     5761 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     5821 cagatggaac agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     5881 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     5941 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     6001 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     6061 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     6121 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
     6181 gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     6241 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6301 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     6361 agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg
     6421 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6481 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6541 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6601 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6661 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6721 gcttttgcaa aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt
     6781 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     7501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     8101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     8401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8761 aaaagtgcca cctgacgtct aagaaaccat tattatcat
//
