LOCUS       sequence-204716-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Quarato G, Guy CS, Grace CR, Llambi F, Nourse A, Rodriguez DA, 
            Wakefield R, Frase S, Moldoveanu T, Green DR
  TITLE     Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding 
            Sites Executes Necroptosis.
  JOURNAL   Mol Cell. 2016 Feb 18;61(4):589-601. doi: 
            10.1016/j.molcel.2016.01.011. Epub 2016 Feb 4.
  PUBMED    26853145
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.01.011"; journalName: "Mol Cell"; date: 
            "2016-02-18- 18"; volume: "61"; issue: "4"; pages: "589-601"
COMMENT     Sequence Label: hMLKL-Venus-Mito
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..45
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        145..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             729..904
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    967..1166
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1367..1783
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1713..1735
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1751..1767
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1823..2172
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1831..1849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1867..1885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1903..1921
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1939..1957
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1975..1993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2011..2029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2047..2065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2111..2135
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3610..3612,3613..3615,3616..4326)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3655..3676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3916..3935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4263..4284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4459..4482)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     gap             4809..4819
                     /estimated_length=11
     primer_bind     4844..4863
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4932..5531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4932..4951)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5428..5448
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5712..6137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6240..6262)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6398..6418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6403..6732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6583..6718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6754..6773
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6854..6871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7025..7613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7105..7124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7784..8575,8576..8644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8407..8426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8645..8749)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
       61 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
      121 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctca ataaaagagc ccacaacccc tcactcggcg cgccagtctt ccgatagact
      781 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
      841 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt
      901 catttggggg ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg
      961 ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga
     1021 tgttatgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg
     1081 tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg
     1141 gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg
     1201 atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt
     1261 gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct
     1321 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac
     1381 cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca
     1441 gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt
     1501 taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat
     1561 caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg
     1621 ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
     1681 gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
     1741 tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaattg aagatctcga
     1801 tgaggccctt tcgtcttcac tcgagtttac tccctatcag tgatagagaa cgtatgaaga
     1861 gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag
     1921 aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta
     1981 tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca
     2041 gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggcgcct
     2101 ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt
     2161 tgtcttatac ttggatccat cgataccatg gaaaatttga agcatattat cacccttggc
     2221 caggtcatcc acaaacggtg tgaagagatg aaatactgca agaaacagtg ccggcgcctg
     2281 ggccaccgcg tcctcggcct gatcaagcct ctggagatgc tccaggacca aggaaagagg
     2341 agcgtgccct ctgagaagtt aaccacagcc atgaaccgct tcaaggctgc cctggaggag
     2401 gctaatgggg agatagaaaa gttcagcaat agatccaata tctgcaggtt tctaacagca
     2461 agccaggaca aaatactctt caaggacgtg aacaggaagc tgagtgatgt ctggaaggag
     2521 ctctcgctgt tacttcaggt tgagcaacgc atgcctgttt cacccataag ccaaggagcg
     2581 tcctgggcac aggaagatca gcaggatgca gacgaagaca ggcgagcttt ccagatgcta
     2641 agaagagata atgaaaaaat agaagcttca ctgagacgat tagaaatcaa catgaaagaa
     2701 atcaaggaaa ctttgaggca gtatttacca ccaaaatgca tgcaggagat cccgcaagag
     2761 caaatcaagg agatcaagaa ggagcagctt tcaggatccc cgtggattct gctaagggaa
     2821 aatgaagtca gcacacttta taaaggagaa taccacagag ctccagtggc cataaaagta
     2881 ttcaaaaaac tccaggctgg cagcattgca atagtgaggc agactttcaa taaggagatc
     2941 aaaaccatga agaaattcga atctcccaac atcctgcgta tatttgggat ttgcattgat
     3001 gaaacagtga ctccgcctca attctccatt gtcatggagt actgtgaact cgggaccctg
     3061 agggagctgt tggataggga aaaagacctc acacttggca agcgcatggt cctagtcctg
     3121 ggggcagcac gaggcctata ccggctacac cattcagaag cacctgaact ccacggaaaa
     3181 atcagaagct caaacttcct ggtaactcaa ggctaccaag tgaagcttgc aggatttgag
     3241 ttgaggaaaa cacagacttc catgagtttg ggaactacga gagaaaagac agacagagtc
     3301 aaatctacag catatctctc acctcaggaa ctggaagatg tattttatca atatgatgta
     3361 aagtctgaaa tatacagctt tggaatcgtc ctctgggaaa tcgccactgg agatatcccg
     3421 tttcaaggct gtaattctga gaagatccgc aagctggtgg ctgtgaagcg gcagcaggag
     3481 ccactgggtg aagactgccc ttcagagctg cgggagatca ttgatgagtg ccgggcccat
     3541 gatccctctg tgcggccctc tgtggatgaa atcttaaaga aactctccac cttttctaag
     3601 gcggccgcaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3721 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     3781 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     3841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4081 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     4141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4201 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     4261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4321 tacaagtcta gagaacgctt caaccgctgg ttcctgacgg gcatgactgt ggccggcgtg
     4381 gttctgctgg gctcactctt cagtcggaaa tgagaattct accgggtagg ggaggcgctt
     4441 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4501 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4561 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4621 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4681 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4741 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggnnnn
     4801 nnnnggggnn nnnnnnnnnc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     4861 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     4921 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4981 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5041 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5101 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5161 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5221 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5281 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5341 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5401 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5461 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5521 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     5581 atgcatcggc gtctcgagat atcagtggtc caggctctag ttttgactca acaatatcac
     5641 cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag
     5701 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     5761 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     5821 acagatggaa cagggtcgac cctagagaac catcagatgt ttccagggtg ccccaaggac
     5881 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5941 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     6001 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     6061 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     6121 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
     6181 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6241 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6301 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6361 cagtcacgta gcgatagcgg agtgtagatc cggctgtgga atgtgtgtca gttagggtgt
     6421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6481 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6541 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6721 ggcttttgca aaaagcttac tggcttaact atgcggcatc agagcagatt gtactgagag
     6781 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacac tcaagtcaga
     7081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7441 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     8101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8401 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8761 gaaaagtgcc acctgacgtc taagaaacca
//
