LOCUS       sequence-20473-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(44..168)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(176..192)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(207..648)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(213..236)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    531..648
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(672..692)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(672..690)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cggaagctaa agtgtaaagc ctgggggtgc ctaatgcggc cgccatagtg actggatatg
       61 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
      121 atttatatca ttttacgttt ctcgttcagc ttttttgtac aaacttgtga tgggggatcc
      181 actagttcta gaatccgggg ttttttctcc ttgacgttaa agtatagagg tatattaaca
      241 attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg aaaatgttga
      301 aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa tagatcaaaa
      361 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca
      421 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa
      481 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
      541 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
      601 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta ctagagctcc
      661 agcttttgtt tcctttagtg agggttaatt gcgcgcttg
//
