LOCUS       sequence-204757-        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  TITLE     Potter Lab plasmids
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQUAST-attB
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        417..655
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     602..623
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          792..857
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             987..1007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1279..1413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1316..1335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1370..1389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1435..1504
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1717..1735)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2187..2258,2660..2933,3008..3662,3724..4039,4243..4374,
                     4445..5059)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(5838..6392,6393..6423)
                     /label=P element 5' end
     rep_origin      complement(6658..7246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6738..6757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7417..8208,8209..8277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8040..8059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8278..8382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8450..8468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8506..8528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8628..8647
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatcca acgcgttggg agctctccgg atccgggtaa tcgcttatcc tcggataaac
      301 aattatcctc acgggtaatc gcttatccgc tcgggtaatc gcttatcctc gggtaatcgc
      361 ttatccttaa gcttgatatc gaattgctgc agcccgagcg gagactctag cgagcgccgg
      421 agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa
      481 cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct
      541 gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac
      601 tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag
      661 ggaattggga attcgttaac agatctgcgg ccgcggctcg agggtacctc tagaggatct
      721 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
      781 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
      841 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
      901 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
      961 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     1021 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     1081 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     1141 aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     1201 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1261 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1321 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1381 aaactcatca atgtatctta tcatgtctgg atcgtcgacg atgtaggtca cggtctcgaa
     1441 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
     1501 catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg
     1561 gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg
     1621 acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt
     1681 cacggcgggc atgtcgaagg cctaattcta gtatgtatgt aagttaataa aacccatttt
     1741 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
     1801 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
     1861 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
     1921 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
     1981 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
     2041 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
     2101 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
     2161 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
     2221 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
     2281 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
     2341 gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca
     2401 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
     2461 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     2521 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     2581 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     2641 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     2701 cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg
     2761 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     2821 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     2881 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     2941 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     3001 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     3061 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     3121 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     3181 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     3241 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     3301 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     3361 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     3421 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
     3481 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     3541 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     3601 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     3661 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
     3721 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     3781 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     3841 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     3901 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     3961 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     4021 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     4081 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     4141 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     4201 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     4261 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     4321 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     4381 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     4441 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     4501 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     4561 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     4621 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     4681 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     4741 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     4801 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     4861 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     4921 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     4981 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     5041 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     5101 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     5161 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     5221 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     5281 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     5341 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     5401 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     5461 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     5521 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     5581 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     5641 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     5701 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     5761 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     5821 gaactagaat taattcggct gctgctctaa acgacgcatt tcgtactcca aagtacgaat
     5881 tttttccctc aagctcttat tttcattaaa caatgaacag gacctaacgc acagtcacgt
     5941 tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt gtactcccac
     6001 tggtatagcc ttcttttatc ttttctggtt caggctctat cactttacta ggtacggcat
     6061 ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc aggtgcagcc ttccactgcg
     6121 aatcattaaa gtgggtatca caaatttggg agttttcacc aaggctgcac ccaaggctct
     6181 gctcccacaa ttttctctta atagcacact tcggcacgtg aattaatttt actccagtca
     6241 cagctttgca gcaaaatttg caatatttca ttttttttta ttccacgtaa gggttaatgt
     6301 tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca acaagcaaac gtgcactgaa
     6361 tttaagtgta tacttcggta agcttcggct atcgacggga ccaccttatg ttatttcatc
     6421 atgggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa gcgtctccag
     6481 gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg
     6541 ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga accatgagag
     6601 gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga aggcgttttt
     6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8461 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     8521 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8581 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     8641 gattgtactg agagtgcacc atatgcggtg tgaaataccg caccgaatcg cgcggaacta
     8701 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     8761 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     8821 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     8881 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     8941 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     9001 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
     9061 ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt
     9121 cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc
     9181 agac
//
