LOCUS       sequence-204759-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector expressing Luciferase with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  TITLE     pLenti-EF1a-Luciferase-IRES-Blast-WPRE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EF1a-Luciferase-IRES-Blast-WPRE
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1950..3128
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2181..3119
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3076..3096
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3128..3152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3153..3162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3159..4811
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3225..3243)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(4812..4836)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4837..5411,5412..5414,5415..5423)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5004..5021)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5231..5250
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5424..5822
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(5823..5843)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5856..6444
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5909..5929)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6327..6338)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6532..6765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6837..6958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6874..6893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6928..6947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6998..7133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7153..7172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7154..7172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7182..7199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7182..7198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7191..7213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7340..7795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7427..7446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7637..7658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7821..7925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7926..7994,7995..8786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8144..8163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8957..9545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9446..9465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9699..9716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9833..9854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9869..9874,9875..9892,9893..9899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9907..9923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9912..9934
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9931..9947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9931..9947
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9966..9986
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9968..9986
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgg gctccggtgc ccgtcagtgg gcagagcgca
     1981 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2041 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2101 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2161 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2221 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2281 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2341 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     2401 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     2461 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     2521 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     2581 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     2641 ctcaagctgg ccggcctgct ctggtgcctg gtctcgcgcc gccgtgtatc gccccgccct
     2701 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     2761 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     2821 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     2881 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     2941 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3001 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3061 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3121 tgtcgtgaca agtttgtaca aaaaagcagg ctgccaccat ggaagacgcc aaaaacataa
     3181 agaaaggccc ggcgccattc tatccgctag aggatggaac cgctggagag caactgcata
     3241 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     3301 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
     3361 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     3421 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3481 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg
     3541 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga
     3601 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3661 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc
     3721 gtgacaaaac aattgcactg ataatgaact cctctggatc tactgggtta cctaagggtg
     3781 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg
     3841 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     3901 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     3961 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag
     4021 taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     4081 atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg
     4141 caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag
     4201 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     4261 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag
     4321 gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     4381 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     4441 aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatacc
     4501 aggtggcccc cgctgaattg gagtcgatat tgttacaaca ccccaacatc ttcgacgcgg
     4561 gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4621 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4681 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4741 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt
     4801 ccaaattgta aacccagctt tcttgtacaa agtgggcccc tctccctccc ccccccctaa
     4861 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4921 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4981 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5041 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5101 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5161 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5221 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5281 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5341 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5401 acgatgataa tatggccaca accatggcca agcctttgtc tcaagaagaa tccaccctca
     5461 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     5521 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     5581 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5641 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5701 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5761 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     5821 aacaacttta ttatacatag ttgatcaatt ccgataatca acctctggat tacaaaattt
     5881 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5941 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6001 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6061 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6121 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6181 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6241 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6301 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6361 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6421 tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgcttta agaccaatga
     6481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6541 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6781 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6961 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7081 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     7141 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7201 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7261 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7321 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7741 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8161 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9361 ccacgcttcc cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9721 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9901 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9961 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
