LOCUS       sequence-20476-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..145)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(153..169)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(175..438)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(258..281)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
       61 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      121 gttcagcttt tttgtacaaa cttgtgatgg gggatccact agttctagaa tccgtcgaaa
      181 ctaagttctg gtgttttaaa actaaaaaaa agactaacta taaaagtaga atttaagaag
      241 tttaagaaat agatttacag aattacaatc aatacctacc gtctttatat acttattagt
      301 caagtagggg aataatttca gggaactggt ttcaaccttt tttttcagct ttttccaaat
      361 cagagagagc agaaggtaat agaaggtgta agaaaatgag atagatacat gcgtgggtca
      421 attgccttgt gtcatcattt actccaggca ggttgcatca ctccattgag gttgtgcccg
      481 ttttttgcct gtttgtgccc ctgttctctg tagttgcgct aagagaatgg acctatgaac
      541 tgatggttgg tgaagaaaac aatattttgg tgctgggatt cttttttttt ctggatgcca
      601 gcttaaaaag cgggctccat tatatttagt ggatgccagg aataaactgt tcacccagac
      661 acctacgatg ttatatattc tgtgtaaccc gccccctatt ttgggcatgt acgggttaca
      721 gca
//
