LOCUS       sequence-204762-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PCDH7 transcript variant b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, 
            Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens 
            C, Wistuba II, Minna JD, O'Donnell KA
  TITLE     PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK 
            Signaling by EGFR and KRAS during Lung Tumorigenesis.
  JOURNAL   Cancer Res. 2017 Jan 1;77(1):187-197. doi: 
            10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7.
  PUBMED    27821484
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-16-1267-T"; journalName: "Cancer Res"; date: 
            "2017-01-1- 1"; volume: "77"; issue: "1"; pages: "187-197"
COMMENT     Sequence Label: pLX304-PCDH7-b
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3870..3894)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3896..3937
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3979..4567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4032..4052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4450..4461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4639..4872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4950..5071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4987..5006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5041..5060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5111..5246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5266..5285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5267..5285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5295..5312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5295..5311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5453..5908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5540..5559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5750..5771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5934..6038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6039..6107,6108..6899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6257..6276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7070..7658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7559..7578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7812..7829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7946..7967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7982..7987,7988..8005,8006..8012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8020..8036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8025..8047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8044..8060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8044..8060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8079..8099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8081..8099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8127..8353
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8354..8534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8581..8706
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9199..9432
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9617..9661
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9836..10336
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10255..10273
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10348..10743
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10803..10920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctc cgaattcgcc cttcaccatg ctgaggatgc ggaccgcggg
      661 atgggcgcgc ggctggtgct tgggctgctg cctcctcctg ccgctctcgc tcagcctggc
      721 ggccgccaag cagctcctcc ggtaccggct ggccgaggag ggccccgccg acgtccgcat
      781 cggcaacgtg gcttcagacc tgggcatcgt gaccggatcg ggtgaggtga ctttcagcct
      841 ggagtccggt tccgagtacc tgaagatcga caacctcact ggcgagctga gcacgagcga
      901 gcggcgcatc gaccgcgaga agctgcccca gtgtcagatg atcttcgacg agaacgagtg
      961 cttcctggac ttcgaggtgt cggtgatcgg gccctcgcag agctgggtgg acctgtttga
     1021 gggtcaggtc atcgtgcttg acatcaacga caacacgccc accttcccgt cgcccgtgct
     1081 cacgctcacg gtggaggaga atcggccggt gggcacactt tacctgctgc ccacagccac
     1141 cgaccgcgac ttcggccgca acggcatcga gcgctacgag ctgctccagg agcccggagg
     1201 cggcggcagc ggcggcgaga gccggcgcgc cggggcggcc gacagcgccc cctaccccgg
     1261 gggcggcggg aacggcgcga gcggcggcgg ctcgggaggc tccaagcggc ggctggacgc
     1321 atcagagggc ggcggcggca ccaaccccgg cggccgcagc agcgtgttcg agctgcaggt
     1381 ggcggacacc ccggacggcg agaagcagcc gcagctgatc gtgaaggggg cgctggaccg
     1441 cgagcagcgc gactcctacg agctgaccct gcgagtgcgc gacggcggcg acccgcctcg
     1501 ctcctcgcag gccatcctac gggtcctcat caccgacgtg aacgacaaca gcccccgctt
     1561 cgagaagagc gtgtacgagg ccgacttggc tgagaacagc gccccgggga cccccatcct
     1621 gcaactgcgc gcagccgact tggacgtggg ggtcaacggg cagatcgaat acgtgttcgg
     1681 ggcggccacc gagtcggtga ggcggctgct gcgccttgac gagacgtccg gctggctcag
     1741 cgtcctgcac cggatcgacc gcgaggaggt gaaccagctg cgcttcacgg tcatggcccg
     1801 cgaccgcggg cagcccccca agaccgacaa ggccaccgtg gtccttaaca tcaaagacga
     1861 gaacgacaac gtgccgtcca ttgaaatccg caagattggg cgcatccccc tcaaggacgg
     1921 ggtggccaac gtggccgagg acgttctggt cgacaccccc atcgctctgg tgcaggtgtc
     1981 cgaccgagac caaggcgaga acggggtggt cacctgcacc gtggtgggcg acgtgccctt
     2041 ccagctcaag ccagccagcg acaccgaggg cgaccagaac aagaaaaagt acttcttgca
     2101 cacctcgacc cctctggact atgaggccac ccgggagttc aacgtggtca tcgtggcggt
     2161 ggactcaggc agccccagcc tctcgagcaa caactccctg attgtcaagg tgggagacac
     2221 caacgacaac ccgcccatgt tcggccagtc ggtggtggag gtttacttcc ctgagaacaa
     2281 catcccgggc gagagggtgg ccacggtgct ggcgacagac gcagacagcg gtaagaacgc
     2341 cgagatcgcc tactcgctgg actcctctgt gatggggatc tttgccatcg atcccgattc
     2401 tggggacatc ctggtcaata ccgtgctgga ccgcgagcag actgacaggt atgagtttaa
     2461 agttaacgcc aaagacaaag gcatccccgt gctgcagggc agcactacgg tgattgtgca
     2521 ggtggctgat aaaaatgaca atgaccctaa gtttatgcag gacgtcttca ccttttatgt
     2581 gaaagaaaac ttgcagccca acagccctgt ggggatggtc accgtgatgg atgctgacaa
     2641 ggggcggaat gcagagatga gcctgtacat agaggagaac aataacattt tttctattga
     2701 aaatgacacg gggaccattt actccacaat gtcttttgac cgggaacatc agaccacata
     2761 cactttcaga gtcaaggctg tggatggggg agatcctccc agatctgcca cagctacagt
     2821 ctcgcttttt gtgatggatg aaaatgacaa tgctcccaca gttacccttc ccaaaaacat
     2881 ttcctacact ttactgccac cttcgagtaa tgtcaggaca gtagtagcta cagtgttggc
     2941 aacagacagt gatgatggca tcaatgcaga cctgaactac agcattgtgg gaggaaatcc
     3001 cttcaagctg tttgaaattg atcccactag tggtgtggtt tccttagtgg gaaaactcac
     3061 ccaaaagcat tatggcttgc acaggttggt ggtgcaagtg aatgacagtg ggcagccttc
     3121 ccagtccacc acgactctgg tgcacgtgtt tgtcaatgaa agtgtttcta atgcaactgc
     3181 gattgactcc cagatagcta gaagtttgca catcccactc acccaggata tagctggtga
     3241 cccaagctat gaaattagca aacagagact cagtattgtc attggcgtgg ttgctggcat
     3301 tatgacggtg attctaatca tcttaattgt agtgatggca aggtactgca ggtccaaaaa
     3361 taaaaatggc tatgaagccg gcaaaaaaga tcacgaagac ttttttacac cccaacagca
     3421 tgacaaatct aaaaagccta aaaaggacaa gaaaaacaaa aaatctaagc agcctctcta
     3481 cagcagcatt gtcactgtgg aggcttctaa gccaaatgga cagaggtatg atagtgtcaa
     3541 tgagaagctg tcagacagcc caagcatggg gcgatacagg tccgttaatg gtgggcccgg
     3601 cagtcctgac ctggcaaggc attacaaatc tagttcccca ttgcctactg ttcagcttca
     3661 tccccagtca ccaactgcag gaaaaaaaca ccaggccgta caagatctac caccagccaa
     3721 cacatttgtg ggagcaggag acaacatttc aattggatca gatcactgct ctgagtacag
     3781 ctgtcaaacc aataacaagt acagcaaaca ggtaagatgt atcccaaata tatttaaata
     3841 tcccagggag ggctaaaagg gcgaattcga cccagctttc ttgtacaaag tggttggtaa
     3901 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg
     3961 gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4381 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4561 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4621 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4681 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4741 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4801 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4861 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4921 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5221 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5281 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5341 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5401 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5881 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6961 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7201 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7861 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8041 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8101 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     8161 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     8221 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     8281 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8341 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8521 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8581 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8641 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8701 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8761 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8821 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8881 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8941 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     9001 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     9061 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9121 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9181 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9241 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9301 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9361 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9421 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9481 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9541 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9601 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9661 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9721 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9781 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg
     9841 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     9901 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     9961 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
    10021 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
    10081 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
    10141 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
    10201 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
    10261 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
    10321 cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc
    10381 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
    10441 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
    10501 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
    10561 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
    10621 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
    10681 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
    10741 ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac
    10801 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    10861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    10921 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg gg
//
