LOCUS       sequence-204767-        2294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis 
            auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS 
            terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, 
            Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara 
            S, Torii KU
  TITLE     Chemical hijacking of auxin signaling with an engineered auxin-TIR1 
            pair.
  JOURNAL   Nat Chem Biol. 2018 Mar;14(3):299-305. doi: 10.1038/nchembio.2555. 
            Epub 2018 Jan 22.
  PUBMED    29355850
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2555"; journalName: "Nat Chem Biol"; date: 
            "2018-03"; volume: "14"; issue: "3"; pages: "299-305"
COMMENT     Sequence Label: pAN19-3xFLAG-ccvTIR1-NOSt
FEATURES             Location/Qualifiers
     source          1..2294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             95..160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      1957..2209
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2234..2251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2234..2250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2243..2265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttcacacagg aaacagctat gaccatgagg cgcgccgcgg ccgcaagctt gcatgcctgc
       61 aggtcgactc tagaggatcc cccgggccgc aatggactac aaagaccatg acggtgatta
      121 taaagatcat gacatcgatt acaaggatga cgatgacaag atgcagaagc gaatagcctt
      181 gtcgtttcca gaagaggtac tagagcatgt gttctcgttt attcagctgg ataaggatag
      241 gaactcagtc tctctggtgt gcaagtcatg gtacgagatc gagcggtggt gcaggaggaa
      301 agtcttcatc gggaactgct acgccgtgag tccagcgacg gtgattagga ggttcccgaa
      361 agtgagatcc gtggagctta aaggaaaacc tcacggtgct gactttaatt tggtacctga
      421 cggatgggga ggttacgtgt atccatggat tgaggccatg tcttcgtctt acacgtggct
      481 tgaagagata aggctgaaga ggatggtggt caccgacgat tgcttggagc tcatagccaa
      541 gtcttttaag aattttaagg tactagtgct ttcttcctgc gaaggcttct ccaccgatgg
      601 tctcgctgct atcgctgcca cttgcaggaa tctgaaagag cttgacttac gagagagtga
      661 tgttgacgac gttagtggcc actggcttag ccatttccca gatacataca cttctttggt
      721 atcactcaat atatcttgct tagcatctga ggtcagtttc tctgctctgg aaaggctggt
      781 gactaggtgt cccaatctca agtctctcaa gcttaaccga gctgttccac ttgaaaaatt
      841 ggctacttta cttcaaagag cacctcaatt ggaggaattg ggcactggtg ggtacactgc
      901 agaagtgcga ccagatgttt actctggttt atctgtagcg ctctctgggt gcaaggaatt
      961 gaggtgctta tctggatttt gggatgctgt tcctgcctat cttccagcag tttattcggt
     1021 ttgcagtcgg cttacaactt tgaatctgag ttatgcaaca gtccagagct atgatcttgt
     1081 caagcttctt tgtcaatgcc ctaaactgca gcgcctctgg gtgcttgact acatcgagga
     1141 tgctggtctt gaggtgcttg cttcaacctg caaggaccta cgcgagctga gagtgtttcc
     1201 gtccgagcct tttgtcatgg aaccaaatgt ggcattgacg gaacaggggc ttgtctccgt
     1261 ctccatgggc tgtccaaaac tcgagtcggt tctctacttc tgccgtcaaa tgaccaatgc
     1321 tgcattgata accattgcta ggaaccgtcc caacatgact cgcttccgtt tgtgcatcat
     1381 tgagccaaaa gccccagact atctgactct agagccactg gatattggat ttggagccat
     1441 agtagagcac tgcaaggatc tccgtcgcct ctctctatct ggcctcttga ccgacaaggt
     1501 ttttgaatac attgggacat atgccaagaa gatggaaatg ctctcagtgg catttgcagg
     1561 agacagtgac ttaggcatgc atcatgtttt gtccgggtgc gatagcttga ggaaactaga
     1621 gataagggac tgcccgtttg gagacaaggc gcttttggcc aatgcttcaa agctggagac
     1681 aatgcgatcc ctttggatgt cttcttgttc cgtgagtttt ggagcctgca agttactagg
     1741 acagaagatg ccaaagctga atgtggaagt catcgatgaa cggggtgcac cggactcgag
     1801 accagagagc tgccctgttg agagagtctt catataccga acagtggctg gtcctcgatt
     1861 tgacatgcct ggcttcgtct ggaacatgga ccaagactca acaatgaggt tttccaggca
     1921 aatcattact actaacggat tataagagct ctccccgatc gttcaaacat ttggcaataa
     1981 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     2041 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2101 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2161 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg aattcggcgc
     2221 gccgcggccg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2281 cccaacttaa tcgc
//
