LOCUS       sequence-204768-        4058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector including C-terminally GUS-tagged Arabidopsis auxin 
            receptor TIR1 [Wild type] and NOS terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, 
            Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara 
            S, Torii KU
  TITLE     Chemical hijacking of auxin signaling with an engineered auxin-TIR1 
            pair.
  JOURNAL   Nat Chem Biol. 2018 Mar;14(3):299-305. doi: 10.1038/nchembio.2555. 
            Epub 2018 Jan 22.
  PUBMED    29355850
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2555"; journalName: "Nat Chem Biol"; date: 
            "2018-03"; volume: "14"; issue: "3"; pages: "299-305"
COMMENT     Sequence Label: pAN19-TIR1-GUS-NOSt
FEATURES             Location/Qualifiers
     source          1..4058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1898..3709
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3721..3973
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3998..4015)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3998..4014)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4007..4029)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttcacacagg aaacagctat gaccatgagg cgcgccgcgg ccgcaagctt gcatgcctgc
       61 aggtcgactc tagaggatcc cccgggccgc aatgcagaag cgaatagcct tgtcgtttcc
      121 agaagaggta ctagagcatg tgttctcgtt tattcagctg gataaggata ggaactcagt
      181 ctctctggtg tgcaagtcat ggtacgagat cgagcggtgg tgcaggagga aagtcttcat
      241 cgggaactgc tacgccgtga gtccagcgac ggtgattagg aggttcccga aagtgagatc
      301 cgtggagctt aaaggaaaac ctcactttgc tgactttaat ttggtacctg acggatgggg
      361 aggttacgtg tatccatgga ttgaggccat gtcttcgtct tacacgtggc ttgaagagat
      421 aaggctgaag aggatggtgg tcaccgacga ttgcttggag ctcatagcca agtcttttaa
      481 gaattttaag gtactagtgc tttcttcctg cgaaggcttc tccaccgatg gtctcgctgc
      541 tatcgctgcc acttgcagga atctgaaaga gcttgactta cgagagagtg atgttgacga
      601 cgttagtggc cactggctta gccatttccc agatacatac acttctttgg tatcactcaa
      661 tatatcttgc ttagcatctg aggtcagttt ctctgctctg gaaaggctgg tgactaggtg
      721 tcccaatctc aagtctctca agcttaaccg agctgttcca cttgaaaaat tggctacttt
      781 acttcaaaga gcacctcaat tggaggaatt gggcactggt gggtacactg cagaagtgcg
      841 accagatgtt tactctggtt tatctgtagc gctctctggg tgcaaggaat tgaggtgctt
      901 atctggattt tgggatgctg ttcctgccta tcttccagca gtttattcgg tttgcagtcg
      961 gcttacaact ttgaatctga gttatgcaac agtccagagc tatgatcttg tcaagcttct
     1021 ttgtcaatgc cctaaactgc agcgcctctg ggtgcttgac tacatcgagg atgctggtct
     1081 tgaggtgctt gcttcaacct gcaaggacct acgcgagctg agagtgtttc cgtccgagcc
     1141 ttttgtcatg gaaccaaatg tggcattgac ggaacagggg cttgtctccg tctccatggg
     1201 ctgtccaaaa ctcgagtcgg ttctctactt ctgccgtcaa atgaccaatg ctgcattgat
     1261 aaccattgct aggaaccgtc ccaacatgac tcgcttccgt ttgtgcatca ttgagccaaa
     1321 agccccagac tatctgactc tagagccact ggatattgga tttggagcca tagtagagca
     1381 ctgcaaggat ctccgtcgcc tctctctatc tggcctcttg accgacaagg tttttgaata
     1441 cattgggaca tatgccaaga agatggaaat gctctcagtg gcatttgcag gagacagtga
     1501 cttaggcatg catcatgttt tgtccgggtg cgatagcttg aggaaactag agataaggga
     1561 ctgcccgttt ggagacaagg cgcttttggc caatgcttca aagctggaga caatgcgatc
     1621 cctttggatg tcttcttgtt ccgtgagttt tggagcctgc aagttactag gacagaagat
     1681 gccaaagctg aatgtggaag tcatcgatga acggggtgca ccggactcga gaccagagag
     1741 ctgccctgtt gagagagtct tcatataccg aacagtggct ggtcctcgat ttgacatgcc
     1801 tggcttcgtc tggaacatgg accaagactc aacaatgagg ttttccaggc aaatcattac
     1861 tactaacgga ttagtcgagg gtggctcgac cggtggtatg ttacgtcctg tagaaacccc
     1921 aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg
     1981 tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc
     2041 aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg
     2101 gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt
     2161 cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca
     2221 gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt
     2281 acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt
     2341 gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc
     2401 cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac
     2461 cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc
     2521 caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca
     2581 aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta
     2641 tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg
     2701 cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt
     2761 ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa
     2821 cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac
     2881 ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt
     2941 gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg
     3001 caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca
     3061 cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg
     3121 gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact
     3181 ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt
     3241 ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta
     3301 ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga
     3361 acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga
     3421 tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc
     3481 atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt
     3541 atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa
     3601 agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg
     3661 gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgag agctctcccc
     3721 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3781 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3841 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3901 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3961 atgttactag atcgaattcg gcgcgccgcg gccgctcact ggccgtcgtt ttacaacgtc
     4021 gtgactggga aaaccctggc gttacccaac ttaatcgc
//
