LOCUS       sequence-20477-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(118..242)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(319..340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(580..599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggggagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
       61 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
      121 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      181 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
      241 gtgatgggct gcaggaattc gatatcaagc ttaatggtga gcaagggcga ggagctgttc
      301 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      361 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      421 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      481 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      541 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      601 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      661 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac
      721 aacgtctata tcac
//
