LOCUS       sequence-204773-       10639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Target-AID vector with sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A
  TITLE     Deaminase-mediated multiplex genome editing in Escherichia coli.
  JOURNAL   Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 
            10.1038/s41564-017-0102-6.
  PUBMED    29403014
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 
            10.1038/s41564-017-0102-6."
COMMENT     Sequence Label: pScI_dCas9-CDA_J23119-sgRNA
FEATURES             Location/Qualifiers
     source          1..10639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1905..1927
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1919..1936
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1920..1936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1971..2017)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2025..2654)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAVT"
     CDS             complement(2661..2726)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(2745..2810)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(3018..4601,4602..4604,4605..7091,7092..7094,
                     7095..7121))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             7227..7235
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7240..7953
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAEMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     promoter        8042..8076
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        8097..8172
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(8427..9086)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9001..9020
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(9140..9146,9147..9164,9165..9170))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(9334..10284)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTMKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      10332..10554
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 cgacgcttta tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga
       61 taaggtttat aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt
      121 aacaaatgtt cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg
      181 agtgagaaaa agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata
      241 gacgaagaaa gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt
      301 aacttcgtaa aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa
      361 aaacttaaaa tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata
      421 gctacaacaa gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca
      481 cttaaaatct tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac
      541 cctgaactac taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg
      601 aactttgagc aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg
      661 ggaggtcaat ctatgaaatg cgattaagct ttttctaatt cacataagcg tgcaggttta
      721 aagtacataa aaaatataat gaaaaaaagc atcattatac taacgttata ccaacattat
      781 actctcatta tactaattgc ttattccaat ttcctattgg ttggaaccaa caggcgttag
      841 tgtgttgttg agttggtact ttcatgggat taatcccatg aaacccccaa ccaactcgcc
      901 aaagctctat attgataaaa ataataatag tgggtataat taagttgtta gagaaaacgt
      961 ataaattagg agggattcat gctggtatga acaaaaatat aaaatattct caaaactttt
     1021 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata
     1081 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta
     1141 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa
     1201 aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca
     1261 aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa
     1321 aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca
     1381 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc
     1441 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct
     1501 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt
     1561 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc
     1621 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat
     1681 tgtctatttt taatagttat ctattattta acgggaggaa ataaatgcat aaactgcatc
     1741 ccttaacttg tttttcgtgt acctattttt tgtgaatcgc cattcgccat tcaggctgcg
     1801 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1861 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1921 taaaacgacg gccagtgaat tcgagctcgg tacccggccg caaacaacag ataaaacgaa
     1981 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctgtcaagt aacagcagga
     2041 ctcttagtgg tgtggagtat ttttacctga atcataatgg acaactcgct ccgtcgtttt
     2101 tcagctcgct tcaaagtctt ctcaagccat ctattctcat tcaattgatt gtgcgacgat
     2161 tggatgaata ttttcctgca acattggtag tgttcactta ccattacatt caacccaacc
     2221 ccgttatctc tgagattcca cagcccaatt tgattcctcg catttttctc gtaatagagt
     2281 ttgcaagccc agattttcaa agtgtggccg ttcccccgca gctcctggtt ataccattct
     2341 aagatctttt cagcgcaatc tgcacaagga ctccaggatg agtaccaatt tatcgtgaat
     2401 tgtccggggt tgtcgcgcag gtattcttcg acttttctaa tgctaaagat ttcggcgtga
     2461 atgccacgtt ctgtcccgct ctgtggttta ttcacagcat agccccaaaa acacgctcta
     2521 cgttcacccc gtcgttttaa ttcaaagaga acgtagcatc tatgcgacac ggattttttg
     2581 ttgttgaaaa actgtttctt aaacgtgtag atgtccaact tctcatggat tctcacgtac
     2641 tcagcgtcgg tcatcctaga cttatcgtca tcgtctttgt aatcaatatc atgatccttg
     2701 tagtctccgt cgtggtcctt atagtctccg gactcgagcc tagacttatc gtcatcgtct
     2761 ttgtaatcaa tatcatgatc cttgtagtct ccgtcgtggt ccttatagtc tccggaatac
     2821 ttctccacgt aagggacagg aatcatcccc ctctttcctt cgctgtcctc tgcattccac
     2881 cactgctcct caggcttatc ccggattctc aggatgtctc ctttcttaaa gggaagatcc
     2941 tcttcatcat tcccattaaa gtcaaagagg gctcgcacat actcagcaga acctccacct
     3001 ccagaacctc ctccaccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga
     3061 ccagtgatgg attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt
     3121 ttacgatcaa ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac
     3181 gtaaataaat gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta
     3241 tatgcactaa gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca
     3301 ctgatttgct caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt
     3361 tgttcgttat cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa
     3421 ttcacatatt tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc
     3481 agcatccgtt tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt
     3541 aagtcttttt taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca
     3601 aaggaacttc tttccataat tgtgatccct agtaactctt taacggattt taacttcttc
     3661 gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca
     3721 aaaccaccat atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt
     3781 ctttttggta aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca
     3841 atattgactt ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc
     3901 cagacaattt ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca
     3961 tttgcaagtg taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt
     4021 gcggttgctt tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact
     4081 ttataatcac catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt
     4141 ccaacgacgg catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact
     4201 ttatagaatt ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact
     4261 ttaacctctc gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa
     4321 atttgtgcca catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca
     4381 gctttatcaa gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt
     4441 tgagtgatta acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact
     4501 tcttcacttg gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta
     4561 ttgtctattg aatcgtcttt aaggaaactt tgtggaacaa tggcatcgac atcataatca
     4621 cttaaacgat taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa
     4681 tagagataga gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc
     4741 tgacttccta attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc
     4801 tggccctttt gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta
     4861 tgccgcccca ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt
     4921 ttaatagcag ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca
     4981 gacacttgtg ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc
     5041 ataaaattgc gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat
     5101 tgcttatccc taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg
     5161 cgacgtttaa gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt
     5221 tcctcaatca tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata
     5281 tcttcatttt cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg
     5341 taggtaccta atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa
     5401 cattctattt ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt
     5461 ttgaagagta aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc
     5521 attccttcag taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa
     5581 agcaaactat gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg
     5641 cgttcaataa atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg
     5701 gtaattgttt cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat
     5761 ggaccaacat aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct
     5821 tttaaaaatg gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga
     5881 atttgatggg gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga
     5941 tttagtttca ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat
     6001 ttataaaatt cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat
     6061 tgatcaaaaa agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa
     6121 agagtcaagt cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc
     6181 ttagttattt cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc
     6241 ttagctgcca aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa
     6301 tcatcatcgt aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt
     6361 gatttaaaat taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt
     6421 ttcttctcac cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt
     6481 gcagaaagaa tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat
     6541 tgattgtagg tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt
     6601 aaatctccct caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag
     6661 attaagcgca aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata
     6721 gttggatatt tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt
     6781 tcatgcttct tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca
     6841 tctactttcg ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga
     6901 cgtgtatacc ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg
     6961 tcaaataaaa gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga
     7021 accttgaact ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta
     7081 tttgtgccga tagctaagcc tattgagtat ttcttatcca tttttgcctc ctaaaatggg
     7141 ccctgcaacc attatcaccg ccagaggtaa aatagtcaac acgcacggtg ttagatattt
     7201 atcccttgcg gtgatagatt taacgttaag gaggtatgta tgagcacaaa aaagaaacca
     7261 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa
     7321 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc
     7381 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttaca
     7441 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag
     7501 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt
     7561 tctcatgttc aggcagagat gttctcacct aagcttagaa cctttaccaa aggtgatgcg
     7621 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa
     7681 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt
     7741 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt
     7801 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta
     7861 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc
     7921 gctagtcagt ggcctgaaga gacgtttggc tgatcggcaa ggtgttctgg tcggcgcata
     7981 gctgataaca attgagcaag aatcttcatc gatgcatcag aaaattattt taaatttcct
     8041 cttgacagct agctcagtcc taggtataat gctagcagag acccgggatg gtctcagttt
     8101 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     8161 ccgagtcggt gctttttttg atacttctat tctactctga ctgcaaacca aaaaaacaag
     8221 cgctttcaaa acgcttgttt tatcattttt agggaaatta atctcttaat ccttttatca
     8281 ttctacattt aggcgctgcc atcttgctaa accccgaaaa aaaaaccccg cccctgacag
     8341 ggcggggttt ttttttctag accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
     8401 gcttttagta agccggatcc acgcgtttac gccccgccct gccactcatc gcagtactgt
     8461 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
     8521 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     8581 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     8641 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     8701 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     8761 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     8821 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     8881 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     8941 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     9001 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     9061 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
     9121 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9181 cctaatgcgg cccctctagc taccggtttt ggtcatgaga ttatctctgt gcatatggac
     9241 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     9301 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     9361 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcatg
     9421 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     9481 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
     9541 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     9601 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     9661 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     9721 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     9781 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     9841 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     9901 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     9961 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
    10021 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
    10081 accataagca ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga
    10141 taccgtccgt tctttcattg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
    10201 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
    10261 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
    10321 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
    10381 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
    10441 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
    10501 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
    10561 ttagtcagtt ccgacgtcaa tgccgagcga aagcgagccg aagggtagca tttacgttag
    10621 ataaccccct gatatgctc
//
