LOCUS       sequence-204811-        2745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and 
            tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-A53T
FEATURES             Location/Qualifiers
     source          1..2745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             52..753
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(832..851)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    897..952
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(951..970)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   2367..2401
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa aggccgccac catgagcgag
       61 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
      121 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      181 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
      241 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
      301 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      361 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
      421 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
      481 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
      541 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
      601 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
      661 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
      721 ctccctagca aactggggca caagcttaat taacactcct caggtgcagg ctgcctatca
      781 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
      841 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
      901 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
      961 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     1021 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     1081 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     1141 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     1201 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     1261 tcttctctta tggagatccc tcgacctgca gcccaagctt ggatccccta ggtttctgat
     1321 cctgtgtaga gaccctagtt tgaatgtgct tatagcctat tattacatgt gtaatgttac
     1381 ataaattact taactcggat ttttaatttc atcagctatt taaaatgggc ataatataac
     1441 tatattaaat ggctgttatg aagattaaat aagatgatat gtaaaatgtg ttttttgttt
     1501 gtttgtttgt ttgtctgttt gtttttttga gacagagtct tgctctgtta cccaggctgg
     1561 agtgcagtgg cacaatcttg gctcactgca agttctacct cccgagttca tgccattctc
     1621 ctgcctcagc ccctcccaag tagctgggac tacaggcacc cgccaccacg cctggctaat
     1681 tttttgtatt tttggtagag atggggtttc accatattag ccaggatggt ctcgatctcc
     1741 tgacctcgtg atctgcccac ctcggcctcc caaattgctg ggattacagg catgagccac
     1801 tgcgcccagc ctaaaatgtt tttttacata atgggtgttc agcacatgtt aaagccttct
     1861 ctccatcctt cttccctttt gtttcatggg ttgactgatc tgtctctagt gctgtacttt
     1921 taaagcttct acagttctga attcaaaatt atcttctcac tgggccccgg tgttatctca
     1981 ttcttttttc tcctctgtaa gttgacatgt gatgtgggaa caaaggggat aaagtcatta
     2041 ttttgtgcta aaatcgtaat tggagaggac ctcctgttag ctgggctttc ttctatttat
     2101 tgtggtggtt actggagttc cttcttctag ttttaggata tatatatata tttttttctt
     2161 tccctgaaga tataataata tatatacttc tgaagattga gatttttaaa ttagttgtat
     2221 tgaaaactag ctaatcagca atttaaggct agcttgagac ttatgtcttg aatttgtttt
     2281 tgtaggctcc aaaaccaagg agggagtggt gcatggtgtg acaacaggta agctccattg
     2341 tgcttatatc gaaagatgat atttaaccct agaaagatag tctgcgtaaa attgacgcat
     2401 gcattcttga aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag
     2461 gacatctcag tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca
     2521 ctgattttga actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga
     2581 cttttaagat ttaactcata cgataattat attgttattt catgttctac ttacgtgata
     2641 acttattata tatatatttt cttgttatag atatcaacta gaatgctagt tattaatagt
     2701 aatcaattac ggggtcatta gttcatagcc catatatgga gttcc
//
