LOCUS       sequence-20483-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..239)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(316..337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(577..596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
       61 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
      121 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
      181 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
      241 atgggctgca ggaattcgat atcaagctta atggtgagca agggcgagga gctgttcacc
      301 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      361 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      421 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
      481 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      541 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      601 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      661 ttcaaggagg acggcaacat cct
//
