LOCUS       sequence-204848-        1605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor scafold with dTOMATO positive selection module, and tagBFP 
            negative selection module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-tagBFP-PSM-dTOMATO
FEATURES             Location/Qualifiers
     source          1..1605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDL
                     PSKLGHKLN"
     primer_bind     complement(775..794)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    840..895
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(894..913)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   1260..1294
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
       61 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
      121 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
      181 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
      241 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
      301 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
      361 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
      421 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
      481 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
      541 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
      601 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
      661 gacctcccta gcaaactggg gcacaagctt aattaacact cctcaggtgc aggctgccta
      721 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
      781 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
      841 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
      901 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
      961 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     1021 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     1081 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     1141 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     1201 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggttaac
     1261 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
     1321 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
     1381 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
     1441 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
     1501 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
     1561 tagatatcaa ctagaatgct agttattaat agtaatcaat tacgg
//
